| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Approximately 50 basidiomycete strains were tested whether they produced extracellular polysaccharides, also known as polysaccharide sheath. Some of these strains produced sheath in the presence of wood blocks and/or lignin-derived aromatic compounds. In order to investigate up-regulated and down-regulated proteins under sheath-producing conditions, moreover, fluorescence two-dimensional difference gel electrophoresis was applied to separate the proteins of a lignin-degrading fungus, Ceriporiopsis subvermispora, which produced large amounts of sheath in the presence of vanillin. Over 100 proteins were identified by peptide mass fingerprinting. Using the same fungus and its sheath, on the other hand, I tried to absorb heavy metals as a part of developing a practical method for decontamination.
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