Microorganisms and enzymes implicated in degradation of tetrodotoxin, pufferfish toxin
| Project/Area Number |
26660169
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Aquatic life science
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| Research Institution | Tokyo University of Marine Science and Technology |
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Principal Investigator |
Nagashima Yuji 東京海洋大学, その他部局等, 教授 (40180484)
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| Co-Investigator(Kenkyū-buntansha) |
NAGAI MAKOTO 岐阜医療科学大学, 保健科学科, 准教授 (30460497)
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| Co-Investigator(Renkei-kenkyūsha) |
KOBAYASHI TAKESHI 東京海洋大学, 学術研究院, 准教授 (60242327)
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| Research Collaborator |
KIRIAKE AYA 東京海洋大学, 海洋科学部, 博士研究員
OHTA AKIRA 東京海洋大学, 大学院海洋科学技術研究科, 博士前期課程
OKAYAMA SAKURAKO 東京海洋大学, 大学院海洋科学技術研究科, 博士前期課程
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 水産学 / 生体分子 / フグ毒 / 糠漬け / メタゲノム解析 |
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| Outline of Final Research Achievements |
Tetrodotoxin (TTX) is thought to originate in marine bacteria and widely distributes in aquatic environments. However, there has been no information about degradation of TTX in nature. Therefore, we explored microorganisms involved in degradation of TTX to clarify the toxin-degradation mechanism, focusing on pufferfish ovary fermented with rice bran, Japanese traditional fermented food. It is known by experience that the fermentation of pufferfish ovary remarkably decrease toxicity during process over 2 years. However, the present study demonstrated that TTX was just transferred from ovary to surrounding by salting and fermentation process, suggesting no or less implication of microorganisms in decreasing toxicity. Metagenomic analysis revealed that extremophiles were detected in salt and rice bran during preservation and the microorganisms managed degradation and/or depression of organic substances in fermented pufferfish ovary by interference.
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Report
(3 results)
Research Products
(9 results)
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[Presentation] 海洋動物の毒2015
Author(s)
長島裕二
Organizer
第37回日本中毒学会総会・学術集会
Place of Presentation
和歌山県和歌山市
Year and Date
2015-07-18
Related Report
Invited
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[Presentation] フグの安全性2015
Author(s)
長島裕二
Organizer
平成26年度神奈川県ふぐ包丁師衛生講習会
Place of Presentation
神奈川県横浜市
Year and Date
2015-03-12
Related Report
Invited
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