Further understanding of resident myogenic stem satellite cell activation:inhibitory regulation by nitration of hepatocyte growth factor
| Project/Area Number |
26660218
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Animal production science
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| Research Institution | Kyushu University |
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Principal Investigator |
TATSUMI RYUICHI 九州大学, (連合)農学研究科(研究院), 准教授 (40250493)
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| Co-Investigator(Kenkyū-buntansha) |
水野谷 航 九州大学, (連合)農学研究科(研究院), 助教 (20404056)
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| Research Collaborator |
ANDERSON JUDY E. マニトバ大学, 理学部, 教授
ALLEN Ronald E. アリゾナ大学, 教授
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 骨格筋 / 筋幹細胞 / 衛星細胞 / 活性化 / 筋肥大・再生 / 肝細胞増殖因子HGF / ニトロ化 / 抑制的制御 / 肝細胞増殖因子(HGF) / 活性化抑制 / 肝細胞増殖因子 / 不活性化 / 細胞増殖因子 / 食肉生産 |
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| Outline of Final Research Achievements |
Postnatal muscle growth and regeneration are largely responsible for activation (re-entry to G0 phase) and proliferation of satellite cells, resident myogenic stem cells normally positioned beneath the basal lamina of muscle fibers. We have thus far found a satellite cell activation mechanism that is triggered by mechanical perturbation and centered on hepatocyte growth factor (HGF) release from the extracellular matrix in a NO radical dependent manner. In order to further understand the mechanism, the present study focused on an inhibitory regulation of satellite cell activation. In vitro study demonstrated that the released HGF may be nitrated by excess NO radical production to lose its biological activity. By understanding this “nitration-driving inhibitory scenario”, we will be able to design novel procedures that specifically enhance muscle growth and regeneration.
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| Academic Significance and Societal Importance of the Research Achievements |
適度な伸展刺激をトリガーとしてNO ラジカルが産生され、これが細胞外マトリックスに結合保持されているHGF の遊離を引き起こすことを申請者らの研究グループが既に報告しているので、強い物理刺激により多量のNO ラジカルが産生されると予想している。物理刺激を引き金として衛星細胞がNO/HGF 依存的に活性化する分子機構の他に、過剰なNO ラジカル産生によるHGFの不活化という抑制的な制御機構の存在が示唆される。HGF 以外の細胞増殖因子のニトロ化による活性制御も含め、研究を更に発展させることにより、筋の肥大や創傷治癒を妨げている分子機構が明らかになると期待される。
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Report
(3 results)
Research Products
(21 results)
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[Journal Article] Transmembrane proteoglycans syndecan-2, 4, receptor candidates for the impact of HGF and FGF2 on semaphorin 3A expression in early-differentiated myoblasts2015
Author(s)
Mai-Khoi Q. Do, Naomi Shimizu, Takahiro Suzuki, Hideaki Ohtsubo, Wataru Mizunoya, Mako Nakamura, Shoko Sawano, Mitsuhiro Furuse, Yoshihide Ikeuchi, Judy E. Anderson, Ryuichi Tatsumi
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Journal Title
Physiological Reports
Volume: 3
Issue: 9
Pages: e12553-e12553
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Anti-inflammatory macrophages implicate in regenerative moto-neuritogenesis, by promoting myoblast migration and Sema3A expression.2014
Author(s)
Sakaguchi, S.,* Shono, J.,* Suzuki, T.,* Sawano, S., Anderson, J.E., Do, M.-K.Q., Ohtsubo, H., Mizunoya, W., Nakamura, M., Furuse, M., Ikeuchi, Y., and Tatsumi, R. (*equal contributors)
Organizer
Animal Science Congress 2014 of the Asian-Australian Association of Animal Production Societies
Place of Presentation
Universitas Gadjah Mada (UGM),Indonesia
Year and Date
2014-11-10 – 2014-11-14
Related Report
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[Presentation] Functional interaction between Sema3A and Pax7 in proliferation and differentiation of myoblasts.2014
Author(s)
Mulan, Nakamura, M., Suzuki, T., Mizunoya, W., Ojima, K., Tatsumi, R. and Ikeuchi, Y.
Organizer
International Symposium on Agriculture, Forestry, Environment and Life Sciences in Asia, 2014 (AFELiSA'14)
Place of Presentation
Ladena Condominium, Korea
Year and Date
2014-10-28 – 2014-10-31
Related Report
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[Presentation] M2 macrophages may implicate in regenerative moto-neuritogenesis, by promoting myoblast migration and Sema3A expression.2014
Author(s)
Tatsumi, R.,* Sakaguchi, S.,* Shono, J.,* Suzuki, T.,* Sawano, S., Anderson, J.E., Do, M.-K.Q., Ohtsubo, H., Mizunoya, W., Nakamura, M., Furuse, M., and Ikeuchi, Y. (*equal contributors)
Organizer
2014 FASEB Science Research Conference on “Skeletal Muscle Satellite and Stem Cells”
Place of Presentation
Steamboat Springs, CO, USA
Year and Date
2014-07-20 – 2014-07-25
Related Report
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[Presentation] APOBEC2 deficiency up-regulates myoblast differentiation.2014
Author(s)
Ohtsubo, H., Sato, Y., Suzuki, T., Mizunoya, W., Nakamura, M., Tatsumi, R., and Ikeuchi, Y.
Organizer
2014 FASEB Science Research Conference on “Skeletal Muscle Satellite and Stem Cells”
Place of Presentation
Steamboat Springs, CO, USA
Year and Date
2014-07-20 – 2014-07-25
Related Report
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[Presentation] Sema3A secreted from satellite cells promotes slow-twitch fiber generation.2014
Author(s)
Suzuki, T., Ohya, Y., Ojima, K., Mizunoya, W., Sawano, S., Ohtsubo, H., Nishimatsu, S., Anderson, J. E., Do, M.-K. Q., Nakamura, M., Furuse, M., Ikeuchi, Y., Nohno, T., and Tatsumi, R.
Organizer
2014 FASEB Science Research Conference on “Skeletal Muscle Satellite and Stem Cells”
Place of Presentation
Steamboat Springs, CO, USA
Year and Date
2014-07-20 – 2014-07-25
Related Report
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