Approach to mechanism for post-operative pulmonary vein stenosis: experimental trials for adventitial hemorrhage-associated oxidative stress regulation
Project/Area Number |
26670190
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Experimental pathology
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Research Institution | The University of Tokushima |
Principal Investigator |
SAKASHITA Naomi 徳島大学, 大学院医歯薬学研究部, 教授 (90284752)
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Co-Investigator(Kenkyū-buntansha) |
HORIGUCHI Hidehisa 徳島大学, 大学院医歯薬学研究部, 准教授 (40304505)
NISHITSUJI Kazuchika 徳島大学, 大学院医歯薬学研究部, 助教 (40532768)
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | ヘムオキシゲナーゼ1 / 平滑筋 / 酸化ストレス / マクロファージ / 粥状動脈硬化 / 内膜肥厚 / 血管平滑筋 / 術後性肺静脈狭窄症 / 内膜増殖 |
Outline of Final Research Achievements |
We examined neointimal proliferation under situation of hemeoxygenase-1 (HO-1) insufficiency. Smooth muscle cell with hemin treatment showed oxidative stress, temporal overexpression of ERK, NF-κB, STAT3, HO-1 and smooth muscle cell proliferation/migration. Immunohistochemistry revealed strong immunoreactivity of oxidative stress marker, 8OHDG, to the proliferated neointima of the post-operative stenosed pulmonary vein. However, no HO-1 was detected in pulmonary vein. These data suggest that HO-1 expression in pulmonary vein prevented hemin-induced oxidative stress and smooth muscle proliferation. To address this hypothesis, effect of HO-1 inhibitor ZnPP on hemin-induced smooth muscle proliferation was examined but ZnPP exerted no effect on smooth muscle cells proliferation. Because our hypothesis did not confirmed, we next examined role of HO-1 on atherosclerosis. We found foamy transformed macrophages in atheromatous plaque failed to express HO-1 and may promote plaque rupture.
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Report
(3 results)
Research Products
(26 results)
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[Journal Article] CUL2-mediated clearance of misfolded TDP-43 is paradoxically affected by VHL in oligodendrocytes in ALS.2016
Author(s)
Uchida T, Tamaki Y, Ayaki T, Shodai A, Kaji S, Morimura T, Banno Y, Nishitsuji K, Sakashita N, Maki T, Yamashita H, Ito H, Takahashi R, Urushitani M.
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Journal Title
Scientific Reports
Volume: 6
Issue: 1
Pages: 19118-19136
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Enthalpy-driven interactions with sulfated glycosaminoglycans promote cell membrane penetration of arginine peptides.2016
Author(s)
Takechi-Haraya Y, Nadai R, Kimura H, Nishitsuji K, Uchimura K, Sakai-Kato K, Ka-wakami K, Shigenaga A, Kawakami T, Otaka A, Hojo H, Sakashita N, Saito H
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Journal Title
Biochimica et Biophysica Acta
Volume: -
Issue: 6
Pages: 1339-1349
DOI
Related Report
Peer Reviewed
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[Journal Article] Concomitant accumulation of α-synuclein and TDP-43 in a patient with corticobasal degeneration.2014
Author(s)
Yamashita S, Sakashita N, Yamashita T, Tawara N, Tasaki M, Kawakami K, Komohara Y, Fujiwara Y, Kamikawa M, Nakagawa T, Hirano T, Maeda Y, Hasegawa M, Takeya M, Ando Y
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Journal Title
Journal of Neurology
Volume: 261
Issue: 11
Pages: 2209-2217
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] 肺病変。2015
Author(s)
吉谷信幸、中川雄伸、菰原義弘、竹屋元裕、坂下直実
Organizer
第117回中国四国スライドカンファレンス
Place of Presentation
鳥取大学医学部、米子市、鳥取県
Year and Date
2015-06-27
Related Report
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[Presentation] 頸部皮下腫瘍。2014
Author(s)
中須千春、堀口英久、坂下直実
Organizer
第114回中国四国スライドカンファレンス
Place of Presentation
高知大学医学部、高知県、南国市
Year and Date
2014-06-14
Related Report
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