Establishment of new system for genomic analysis for AML using by desk-top next generation sequencer
Project/Area Number |
26670277
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Laboratory medicine
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Research Institution | Kyoto University |
Principal Investigator |
ADACHI Souichi 京都大学, 医学(系)研究科(研究院), 教授 (10273450)
|
Co-Investigator(Kenkyū-buntansha) |
SHIMADA Akirara 岡山大学, 医学研究科, 講師 (70391836)
|
Project Period (FY) |
2014-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
|
Keywords | 次世代シークエンサー / 予後因子 / AML / 遺伝子検査学 |
Outline of Final Research Achievements |
It is necessary for clinical studies to analyze many genomic genes analysis at the same time by using next-generation sequencer. For the purpose of establishing new methods to analyze many genes quickly and less expensively, we made gene analysis panels for child acute myeloid leukemia patients by desk-top next generation sequencer. We selected 150 genes from DNA samples of AML (primary, remission and relapse) by using Haloplex customized panel of Agilent Co. We analyzed 8 cases of AML and detected IKZF, KRAS, GATA1, KIT, PU.1 mutation in four cases. We also detected large deletions of SH2B3 and SMARCB3 genes in the other four cases. We are going to analyze many samples from JPLSG AML-05 studies by the panel using desk-top next generation sequencer.
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Report
(2 results)
Research Products
(10 results)
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[Journal Article] Adverse Prognostic Impact of KIT Mutations in Childhood CBF-AML: the Results of the Japanese Pediatric Leukemia/Lymphoma Study Group AML-05 Trial.2015
Author(s)
Tokumasu M, Murata C, Shimada A, Ohki K, Hayashi Y, Saito AM, Fujimoto J, Horibe K, Nagao M, Itoh H, Kamikubo Y, Nakayama H, Kinoshita A, Tomizawa D, Taga1 T, Tawa A, Tanaka S, Heike T,and Adachi S
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] High expression of EVI1 and MEL1 is a compelling poor prognostic marker of pediatric AML.2015
Author(s)
Jo A, Mitani S, Shiba N, Hayashi Y, Hara Y, Takahashi H, Tsukimoto I, Tawa A, Horibe K, Tomizawa D, Taga T, Adachi S, Yoshida T, Ichikawa H
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Journal Title
Leukemia
Volume: 29
Issue: 5
Pages: 1067-1083
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] EVI1 overexpression is a poor prognostic factor in pediatric patients with mixed lineage leukemia-AF9 rearranged acute myeloid leukemia.2014
Author(s)
Matsuo H, Kajihara M, Tomizawa D, Watanabe T, Saito AM, Fujimoto J, Horibe K, Kodama K, Tokumasu M, Itoh H, Nakayama H, Kinoshita A, Taga T, Tawa A, Taki T, Shiba N, Ohki K, Hayashi Y, Yamashita Y, Shimada A, Tanaka S, Adachi S.
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Journal Title
Haematologica
Volume: 99
Issue: 11
Pages: 225-227
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Outcome of children with relapsed acute myeloid leukemia following initial therapy under the AML99 protocol2014
Author(s)
Nakayama H, Tabuchi K, Tawa A, Tsukimoto I, Tsuchida M, Morimoto A, Yabe H, Horibe K, Hanada R, Imaizumi M, Hayashi Y, Hamamoto K, Kobayashi R, Kudo K, Shimada A, Miyamura T, Moritake H, Tomizawa D, Taga T, Adachi S.
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Journal Title
Int J Hematol
Volume: 100(2)
Issue: 2
Pages: 171-9
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Prognostic implications of CEBPA mutations in pediatric acute myeloid leukemia: a report from the Japanese Pediatric Leukemia/Lymphoma Study Group.2014
Author(s)
Matsuo H, Kajihara M, Tomizawa D, Watanabe T, Saito AM, Fujimoto J, Horibe K, Kodama K, Tokumasu M, Itoh H, Nakayama H, Kinoshita A, Taga T, Tawa A, Taki T, Tanaka S, Adachi S.
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Journal Title
Blood Cancer J
Volume: 4
Issue: 7
Pages: e226-e226
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Poor prognosis associated with FAB subtypes M4 and M5 in Japanese pediatric acute myeloid leukemia patients with FLT3-ITD.2014
Author(s)
Hara Y, Shiba N, Ohki K, Park M-J, Shimada A, Tomizawa D, Saito AM, Fujimoto J, Taki T, Kinoshita A, Taga T, Arakawa H, Tawa A, Horibe K, Adachi S, Hayashi Y.
Organizer
56th Annual Meeting of the American Society of Hematology
Place of Presentation
San Francisco
Year and Date
2014-12-06 – 2014-12-09
Related Report
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[Presentation] Clinical features of patients with ASXL1 and ASXL2 mutations in pediatric acute myeloid leukemia.2014
Author(s)
Yamato G, Shiba N, Yoshida K, Ohki K, Park M-J, Hara Y, Tomizawa D, Sotomatsu M, Taga T, Adachi S, Tawa A, Horibe K, Arakawa H, Ogawa S, Hayashi Y.
Organizer
56th Annual Meeting of the American Society of Hematology
Place of Presentation
San Francisco
Year and Date
2014-12-06 – 2014-12-09
Related Report
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[Presentation] Pediatric acute megakaryoblastic leukemia without Down syndrome: a retrospective study by the International Berlin-Frankfurt-Munster Study Group (I-BFMSG).2014
Author(s)
Inaba H, Zhou Y, Abla O, Adachi S, Auvrignon A, Beverloo HB, de Bont E, Chang T-T, Creutzig U, Dastugue N, Dworzak M, Elitzur S, Fynn A, Forestier E, Hasle H, Liang D-C, Lee V, Locatelli F, Masetti R, De Moerloose B, Reinhardt D, Rodriguez L, Shen S, Taga T, Tomizawa D, Yeoh AEJ, Zimmermann M, Raimondi SC.
Organizer
56th Annual Meeting of the American Society of Hematology
Place of Presentation
San Francisco
Year and Date
2014-12-06 – 2014-12-09
Related Report
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[Presentation] Pediatric AML with FLT3-ITD/WT, NUP98-NSD1, NPM1, and WT1 mutations affected the clinical outcome.2014
Author(s)
Shimada A, Yamashita Y, Tomizawa D, Shiba N, Tawa A, Watanabe T, Yokozawa T, Kudo K, Taga T, Iwamoto S, Terui K, Moritake H, Kinoshita A, Takahashi H, Nakayama H, Koh K, Goto H, Kosaka Y, Saito AM, Fujimoto J, Horibe K, Hara Y, Oki K, Hayashi Y, Adachi S.
Organizer
第76回日本血液学会総会
Place of Presentation
大阪
Year and Date
2014-10-31 – 2014-11-02
Related Report
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[Presentation] The prognostic impact of high EVI1-related genes expression in pediatric acute myeloid leukemia.2014
Author(s)
Shiba N, Hara Y, Ohki K, Yamato G, Park M-J, Kobayashi T, Ichikawa H, Tomizawa D, Taki T, Shimada A, Sotomatsu M, Arakawa H, Horibe K, Adachi S, Tawa A, Hayashi Y.
Organizer
第76回日本血液学会総会
Place of Presentation
大阪
Year and Date
2014-10-31 – 2014-11-02
Related Report