Integrative omics-based understanding of the appetite-regulating signals within the hypothalamus
Project/Area Number |
26670458
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Endocrinology
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Research Institution | Kyoto University |
Principal Investigator |
KAZUWA Nakao 京都大学, 医学(系)研究科(研究院), 教授 (00172263)
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Co-Investigator(Renkei-kenkyūsha) |
TANAKA Tomohiro 京都大学, 大学院医学研究科, 特定准教授 (20402894)
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Project Period (FY) |
2014-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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Keywords | オミックス / レプチン / 食欲 / 肥満 / シグナル伝達 |
Outline of Final Research Achievements |
We have undertaken phospho-proteomics and metabolomics analyses of the mouse hypothalamus to understand the cross-talk of nutrient signals and phospho-protein signaling. We have established an effective immunoprecipitation method using phospho-tyrosine antibody for the subsequent SDS-PAGE, Ag staining and MS-based annotation. We have already identified multiple novel positive bands in Ag-stained gels that appear by leptin stimulation, which could correspond to novel phosphor-proteins in leptin signaling pathway. In CE/MS-based analysis of the intermediate metabolites in central metabolic pathways revealed a drastic change in nucleic acid and purine metabolism and in pentose-phosphate pathway in hypothalamic samples from mice treated with leptin. Linkage between changes in phosphate group donors and global changes in phospho-proteomics may suggest a novel signaling dynamism of leptin actions in the hypothalamic energy center.
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Report
(2 results)
Research Products
(20 results)
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[Journal Article] Increased bone turnover and possible accelerated fracture healing in a murine model with an increased circulating C-type natriuretic peptide.2015
Author(s)
Kondo E, Yasoda A, Fujii T, Nakao K, Yamashita Y, Ueda-Sakane Y, Kanamoto N, Miura M, Arai H, Mukoyama M, Inagaki N, Nakao K
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Journal Title
Endocrinology
Volume: Apr 10
Pages: 00-00
Related Report
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[Journal Article] Leptin improves fatty liver independently of insulin sensitization and appetite suppression in hepatocyte-specific Pten-deficient mice with insulin hypersensitivity.2015
Author(s)
Yamamoto-Kataoka S, Ebihara K, Aizawa-Abe M, Nishio M, Kusakabe T, Yamamoto Y, Aotani D, Sakai T, Zhao M, Ebihara C, Gumbilai VM, Hosoda K, Suzuki A, Nakao K
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Journal Title
Horm Metab Res
Volume: 47
Issue: 03
Pages: 168-175
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] An AKI biomarker lipocalin 2 in the blood derives from the kidney in renal injury but from neutrophils in normal and infected conditions.2015
Author(s)
Kanda J, Mori K, Kawabata H, Kuwabara T, Mori KP, Imamaki H, Kasahara M, Yokoi H, Mizumoto C, Thoennissen NH, Koeffler HP, Barasch J, Takaori-Kondo A, Mukoyama M, Nakao K.
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Journal Title
Clin Exp Nephrol.
Volume: 19
Issue: 1
Pages: 99-106
DOI
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Role of endogenous ACTH on circadian aldosterone rhythm in patients with primary aldosteronism2014
Author(s)
Sonoyama, T., Sone, M. , Tamura, N., Honda, K., Taura, D., Kojima, K., Fukuda, Y., Kanamoto, N., Miura, M., Yasoda, A., Arai, H., Itoh, H., Nakao, K
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Journal Title
Endocrine Connections
Volume: 3
Issue: 4
Pages: 173-179
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Leptin restores the insulinotropic effect of exenatide in a mouse model of type 2 diabetes with increased adiposity induced by streptozotocin and high-fat diet2014
Author(s)
T. Sakai, T. Kusakabe, K. Ebihara, D. Aotani, S. Yamamoto-Kataoka, Z. Mingming, VM. Gumbilai, C. Ebihara, M. Aizawa-Abe, Y. Yamamoto, M. Noguchi, J. Fujikura, K. Hosoda, N. Inagaki, K. Nakao
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Journal Title
Am J Physiol Endocrinol Metab.
Volume: 307
Issue: 8
Pages: 712-719
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Inhibition of N-type Ca2+ channels ameliorates an imbalance in cardiac autonomic nerve activity and prevents lethal arrhythmias in mice with heart failure.2014
Author(s)
Yamada Y, Kinoshita H, Kuwahara K, Nakagawa Y, Kuwabara Y, Minami T, Yamada C, Shibata J, Nakao K, Cho K, Arai Y, Yasuno S, Nishikimi T, Ueshima K, Kamakura S, Nishida M, Kiyonaka S, Mori Y, Kimura T, Kangawa K, Nakao K.
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Journal Title
Cardiovasc Research
Volume: 104
Issue: 1
Pages: 183-193
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] The effects of super-flux (high performance) dialyzer on plasma glycosylated pro-B-type natriuretic peptide (proBNP) and glycosylated N-Terminal proBNP in end-stage renal disease patients on dialysis.2014
Author(s)
Nakagawa Y, Nishikimi T, Kuwahara K, Yasuno S, Kinoshita H, Kuwabara Y, Nakao K, Minami T, Yamada C, Ueshima K, Ikeda Y, Okamoto H, Horii K, Nagata K, Kangawa K, Minamino N, Nakao K.
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Journal Title
PLoS One
Volume: 25
Issue: 3
Pages: e92314-e92314
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Macrophage-mediated glucolipotoxicity via myeloid-related protein 8/toll-like receptor 4 signaling in diabetic nephropathy2014
Author(s)
Kuwabara, T., Mori, K., Mukoyama, M., Kasahara, M., Yokoi, H., Nakao, K.
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Journal Title
Clinical and Experimental Nephrology
Volume: impress
Issue: 4
Pages: 584-592
DOI
NAID
Related Report
Peer Reviewed
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