Comprehensive Analysis of the Regulation of Basophil Development

• Project/Area Number: 26670502
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pediatrics
• Research Institution: The University of Tokushima
• Principal Investigator: MINEGISHI Yoshiyuki 徳島大学, 疾患プロテオゲノム研究センター, 教授 (10343154)
• Co-Investigator(Renkei-kenkyūsha): NISHIKAWA Yumiko 徳島大学, 疾患プロテオゲノム研究センター, 助教 (60448214) ; WADA Takeshi 徳島大学, 疾患プロテオゲノム研究センター, 特任助教 (80583418)
• Research Collaborator: MINEGISHI Shizuko ; INOUE Fumiko
• Project Period (FY): 2014-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: アレルギー

Outline of Final Research Achievements

Hyper-IgE syndrome is one of the primary immunodeficiency, caused by dominant negative mutations of the STAT3 gene (Minegishi et al. Nature 2007). Most of the patients with this disorder suffer from severe atopic dermatitis, but the mechanistic basis is still elusive. To elucidate a molecular mechanism, we established a model mice of this syndrome and developed an atopic dermatitis model with the mice. We found that atopic dermatitis is more severe in Stat3 dominant negative mice compared to wild-type mice. We investigate the molecular mechanism of this phenotype.

Research Products

• [Journal Article] 原発性免疫不全症の原因遺伝子探索の新展開 (2015)
  • Author(s): 峯岸克行
  • Journal Title: 医学のあゆみ Volume: 252 Pages: 5-9
• [Journal Article] 高IgE症候群 (2015)
  • Author(s): 峯岸克行
  • Journal Title: 臨床免疫アレルギー科 Volume: 63 Pages: 251-253
• [Journal Article] Temporal lineage tracing of Aire-expressing cells reveals a requirement for Aire in their maturation program (2014)
  • Author(s): Nishikawa, Y., Nishijima, H., Matsumoto, M., Morimoto, J., Hirota, F., Takahashi, S., Luche, H., Fehling, H.J., Mouri, Y., Matsumoto, M.
  • Journal Title: J. Immunol Volume: 192 Issue: 6 Pages: 2585-2592
  • DOI: 10.4049/jimmunol.1302786
  • Peer Reviewed / Open Access
• [Journal Article] Simple diagnosis of STAT1 gain-of-function alleles in patients with chronic mucocutaneous candidiasis. (2014)
  • Author(s): Mizoguchi Y, Tsumura M, Okada S, Hirata O, Minegishi S, Imai K, Hyakuna N, Muramatsu H, Kojima S, Ozaki Y, Imai T, Takeda S, Okazaki T, Ito T, Yasunaga S, Takihara Y, Bryant VL, Kong XF, Cypowyj S, Boisson-Dupuis S, Puel A, Casanova JL, Morio T, Kobayashi M.
  • Journal Title: J. Leukoc. Biol. Volume: 95 Issue: 4 Pages: 667-76
  • DOI: 10.1189/jlb.0513250
  • Peer Reviewed / Open Access
• [Journal Article] 高IgE症候群の病態形成メカニズム (2014)
  • Author(s): 峯岸克行
  • Journal Title: 炎症と免疫 Volume: 22 Pages: 18-22
• [Presentation] Specific DSB induction to STAT3 mutations by CRISPR/Cas9 (2015)
  • Author(s): Minegishi S, Urabe K, F Inoue, Y Minegishi
  • Organizer: Keystone symposium “Precision Genome Engineering and Synthetic Biology
  • Place of Presentation: Big Sky MN, USA
  • Year and Date: 2015-01-11 – 2015-01-17
• [Presentation] ヒト遺伝性アレルギー疾患｢高IgE症候群｣の発症機構の解明とその制御 (2014)
  • Author(s): 峯岸克行
  • Organizer: 第3回CREST免疫機構領域シンポジウム
  • Place of Presentation: 東京医科歯科大学大講堂（東京都文京区）
  • Year and Date: 2014-10-08
• [Presentation] 高IgE症候群の病因と病態の解明 (2014)
  • Author(s): 峯岸克行
  • Organizer: 第21回日本免疫毒性学会学術年会
  • Place of Presentation: 徳島文理大学・国際会議場（徳島県徳島市）
  • Year and Date: 2014-09-11
  • Invited