Development of iPSC-derived T cells with antigen-specific helper or regulatory function
| Project/Area Number |
26670578
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
General surgery
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
Kaneko Shin 京都大学, iPS細胞研究所, 准教授 (40361331)
|
|---|
| Project Period (FY) |
2014-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | iPS細胞 / 再分化T細胞 / CD4T細胞 / T-iPS細胞 / 免疫再生 / 免疫制御 / 同種移植 / T細胞分化 / 移植免疫 / 抗原特異的T細胞 / 再生T細胞 |
|---|
| Outline of Final Research Achievements |
Three different antigen specific CD4 T cell clones are isolated from human peripheral blood and reprogrammed into T-iPS cells. For the purpose of induce HLA Class II restricted TCR expressing antigen specific T cells with helper function or regulatory function, current T cell differentiation protocol from iPS cell was optimized. Those T cells induced by the protocol showed HLA-class II restricted antigen specific proliferation and cytokine production. In addition, such HLA Class II restricted TCR expressing re-differentiated T cells showed an adjuvant function to induce antigen-specific CD8 CTLs for desired antigen via matulation of dendritic cells. The adjuvant effect was similar to teh effect induced by Type I helper T cells.
|
| Academic Significance and Societal Importance of the Research Achievements |
がん治療における免疫反応の重要性は広く知られるところである。iPS細胞は抗原特異的CD8キラーT細胞再生のソースとして期待されているが、抗原特異的CD4の再生についての報告はない。本研究では、抗原特異的TCRを発現しヘルパー機能をもつCD4発現細胞をiPS細胞から誘導することに成功した。キラーT細胞との併用により、治療効果の向上が期待できる。
|
Report
(3 results)
Research Products
(46 results)
-
[Journal Article] Cellular adjuvant properties and direct cytotoxicity of redifferentiated Vα24 invariant NKT-like cells from human induced pluripotent stem cells2016
Author(s)
Kitayama S, Zhang R, Liu TY, Ueda N, Iriguchi S, Yasui Y, Kawai Y, Tatsumi M, Hirai N, Mizoro Y, Iwama T, Watanabe A, Nakanishi M, Kuzushima K, Uemura Y, Kaneko S
-
Journal Title
Stem Cell Reports.
Volume: 6
Pages: 213-227
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
-
[Journal Article] Hepatocellular carcinoma cell sensitivity to Vγ9Vδ2 T lymphocyte-mediated killing is increased by zoledronate2016
Author(s)
Sugai S, Yoshikawa T, Iwama T, Tsuchiya N, Ueda N, Fujinami N, Shimomura M, Zhang R, Kaneko S, Uemura Y, Nakatsura T.
-
Journal Title
Int. J. Oncol.
Volume: 48(5)
Pages: 1794-1804
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
[Journal Article] BCR-ABL-specific CD4+ T helper cells promote the priming of antigen-specific cytotoxic T cells via dendritic cells.2016
Author(s)
Ueda N, Zhang R, Tatsumi M, Liu TY, Kitayama S, Yasui Y, Sugai S, Senju S, Kuzushima K, Kiyoi H, Kaneko S, Uemura Y.
-
Journal Title
Cellular and Molecular Immunology
Volume: In Press
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Generation of BCR-ABL Reactive CD4+ T Helper Cells By Reprograming and Redifferentiation.2015
Author(s)
Ueda N.,Uemura Y., Rhong Z., Kitayama S., Yasui Y., Tatsumi M., Liu T, Y., Kuzushima K., Kiyoi H., Naoe T., Kaneko S.
Organizer
The 57th ASH Annual Meeting and Exposition
Place of Presentation
Orland,USA
Year and Date
2015-12-07
Related Report
Int'l Joint Research
-
-
-
[Presentation] Generation of BCR-ABL reactive CD4 T lymphocytes by reprograming and redifferentiation.2015
Author(s)
Ueda N.,Uemura Y., Rhong Z., Kitayama S., Yasui Y., Tatsumi M., Liu T, Y., Kuzushima K., Kiyoi H., Naoe T., Kaneko S.
Organizer
第44回日本免疫学会学術総会
Place of Presentation
札幌
Year and Date
2015-11-19
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
