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Epigenetic abnormality induced by aberrant c-Myc expression leads to the chromosomal instability.

Research Project

Project/Area Number 26670668
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Orthopaedic surgery
Research InstitutionOita University

Principal Investigator

ichiro itonaga  大分大学, 医学部, 講師 (10295181)

Co-Investigator(Kenkyū-buntansha) TANAKA Kazuhiro  大分大学, 医学部人工関節講座, 講師 (10274458)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords肉腫 / 染色体不安定性 / c-myc / 染色体転座 / エピジェネティクス / cmyc
Outline of Final Research Achievements

By forcing c-Myc expression and performing ChIP-sequence in that state, we have analyzed and verified data by microRNA and cDNA array. Currently it is likely that let-7a, miR-16, miR-29b, miR-1294, and/or miR-2682 are involved. We demonstrated that the transfection of these two factors suppresses the expression of mRNA and protein on c-Myc, and also suppresses the progression of the cell cycle.To examine the presence or absence of chromosomal instability during the study period, we are attempting to detect abnormalities in genome fraction and in the number of DNA copies using the DNA ploidy analysis and array-CGH. Our prediction is that one of the DSB repair systems, homologous recombination or non-homologous end joining, has a greater dysfunction. We are seeking to determine this through HR activity measurement in c-myc-introduced fibroblasts by I-Scel, and random plasmid integration, which is an NHEJ assay.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (12 results)

All 2016 2015 2014

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (4 results)

  • [Journal Article] MicroRNA-301a promotes cell proliferation via PTEN targeting in Ewing's sarcoma cells.2016

    • Author(s)
      Kawano M, Tanaka K, Itonaga I, Iwasaki T, Tsumura H.
    • Journal Title

      International Jnournal of Oncology.

      Volume: Apr;48(4): Issue: 4 Pages: 1531-1540

    • DOI

      10.3892/ijo.2016.3379

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tumor suppressive microRNA-138 inhibits metastatic potential via the targeting of focal adhesion kinase in Ewing's sarcoma cells.2016

    • Author(s)
      Kawano M, Tanaka K, Itonaga I, Iwasaki T, Miyazaki M, Ikeda S, Tsumura H.
    • Journal Title

      International Jnournal of Oncology.

      Volume: Mar;48(3): Issue: 3 Pages: 1135-1144

    • DOI

      10.3892/ijo.2016.3317

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dendritic Cells Combined with Immunogenic Cell Death Induced by Doxorubicin Produces Antitumor Effects in Osteosarcoma.2016

    • Author(s)
      Kawano M, Tanaka K, Itonaga I, Iwasaki T, Miyazaki M, Ikeda S, Tsumura H.
    • Journal Title

      Oncology Letters.

      Volume: 11 Pages: 2169-2175

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] c-MYc represses tumor-suppressive microRNAs, let-7a, miR-16 and miR-29b, and induces cyclin D2-mediated cell proliferation in Ewing’s sarcoma cell line.2015

    • Author(s)
      Kawano M, Tanaka K, Itonaga I, Iwasaki T, Tsumura H
    • Journal Title

      Plos One

      Volume: 10 Issue: 9 Pages: e0138560-e0138560

    • DOI

      10.1371/journal.pone.0138560

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] microRNA-93 promotes cell proliferation via targeting of PTEN in osteosarcoma cells.2015

    • Author(s)
      Kawano M, Tanaka K, Itonaga I, Ikeda S, Iwasaki T, Tsumura H
    • Journal Title

      J Exp Clin Cancer Res

      Volume: 34 Issue: 1 Pages: 76-76

    • DOI

      10.1186/s13046-015-0192-z

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Tumor-suppressive microRNA-let-7a inhibits cell proliferation via targeting of E2F2 in osteosarcoma cells.2015

    • Author(s)
      Iwasaki T, Tanaka K, Kawano M, Itonaga I, Tsumura H
    • Journal Title

      Int J Oncol

      Volume: 46 Issue: 4 Pages: 1543-1550

    • DOI

      10.3892/ijo.2015.2867

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Dendrotic cells combined with anti-GITR antibody produce antitumor effects in osteosarcoma.2015

    • Author(s)
      Kawano M, Tanaka K, Itonaga I, Iwasaki T, Miyazaki M, Ikeda S, Tsumura H
    • Journal Title

      Oncol Rep

      Volume: 34 Issue: 4 Pages: 1995-2001

    • DOI

      10.3892/or.2015.4161

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dendritic Cells Combined with Immunogenic Cell Death Induced by Doxorubicin Exhibits Antitumor Effects for Osteosarcoma2015

    • Author(s)
      M. Kawano, K. Tanaka, I. Itonaga, T. Iwasaki, M. Miyazaki, S. Ikeda, H. Tsumura
    • Journal Title

      Oncology Leteers

      Volume: in press

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] microRNA-93 promotes cell proliferation via targeting of PTEN in Osteosarcoma cells.2015

    • Author(s)
      M Kawano, K Tanaka, I. Itonaga, T. Iwasaki, H. Tsumura
    • Organizer
      Annual research meeting of the Japanese orthopedic association
    • Place of Presentation
      富山国際会議場(富山県、富山市)
    • Year and Date
      2015-10-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] microRNA-301a promotes cell proliferation via targeting of PTEN in Ewing’s sarcoma cells2015

    • Author(s)
      M Kawano, K Tanaka, I. Itonaga, T. Iwasaki, H. Tsumura.
    • Organizer
      Japan Muscular Skeletal Tumor Society Meeting
    • Place of Presentation
      かがわ国際会議場(香川県、高松市)
    • Year and Date
      2015-07-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] 当科におけるパゾパニブの臨床使用経験2014

    • Author(s)
      糸永一朗
    • Organizer
      日本整形外科学会骨・軟部腫瘍学術集会
    • Place of Presentation
      大阪国際会議場(大阪府、大阪市)
    • Year and Date
      2014-07-17 – 2014-07-18
    • Related Report
      2014 Research-status Report
  • [Presentation] 関節症状なく足底にリウマトイド結節が生じた1例2014

    • Author(s)
      糸永一朗
    • Organizer
      日本リウマチ学会総会
    • Place of Presentation
      グランドプリンスホテル新高輪(東京都港区)
    • Year and Date
      2014-04-24 – 2014-04-26
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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