The synthesis of new compounds that reveals anti-bacterial biofilm activity

• Project/Area Number: 26670816
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Niigata University
• Principal Investigator: Terao Yutaka 新潟大学, 医歯学系, 教授 (50397717)
• Co-Investigator(Kenkyū-buntansha): ODA Masataka 新潟大学, 医歯学系, 准教授 (00412403) ; DOMON Hisanori 新潟大学, 医歯学系, 助教 (00594350)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 口腔細菌 / バイオフィルム阻害 / 脂質化合物

Outline of Final Research Achievements

We have been investigated (2R, 3R)-trehalose-6,6′-dicorynomycolate, and we named the material as a “Vizantin”. It revealed immunostimulating properties and anti-cancer response in vitro assays. In this present Challenging Explpratory Research, we synthesized or modified the insoluble Vizantin to soluble analogues, and then we used them for the analyses to search anti-bacterial biofilm molecules. The biochemical and imaging assays demonstrated that one of the Vizantin analogues inhibited Streoptococcus mutans biofilm formation.

Research Products

• [Journal Article] Poor survival of Methicillin-resistant Staphylococcus aureus on inanimate objects in the public spaces. (2016)
  • Author(s): Domon H, Uehara Y, Oda M, Seo H, Kubota N, Terao Y:
  • Journal Title: MicrobiologyOpen Volume: 5 Pages: 39-46
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Streptococcus pyogenes CAMP factor attenuates phagocytic activity of RAW 264.7 cells. (2016)
  • Author(s): Kurosawa M, Oda M, Domon H, Saitoh I, Hayasaki H, Terao Y:
  • Journal Title: Microb Infect Volume: 18 Pages: 118-127
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Anti-biofilm and bactericidal effects of Magnolia bark-derived magnolol and honokiol on Streptococcus mutans. (2016)
  • Author(s): Sakaue Y, Domon H, Oda M, Takenaka S, Kubo M, Fukuyama Y, Okiji T, Terao Y:
  • Journal Title: Microbiol Immunol Volume: 60 Pages: 10-16
  • NAID: 40020725379
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Chemical hybridization of Vizantin and Lipid A to generate a novel LPS antagonist. (2016)
  • Author(s): Yamamoto H, Oda M, Kanno M, Tamashiro S, Tamura I, Yoneda T, Yamasaki N, Domon H, Nakano M, Takahashi H, Terao Y, Kasai Y, Imagawa H:
  • Journal Title: Chem Pham Bull Volume: 64 Pages: 246-257
  • NAID: 130005131585
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Clostridium perfringens alpha-toxin induces GM1a clustering and TrkA phosphorylation in the host cell membrane (2015)
  • Author(s): Takagishi T, Oda M, Kabura M, Kurosawa M, Tominaga K, Urano S, Ueda Y, Kobayashi K, Kobayashi T, Sakurai J, Terao Y, Nagahama M
  • Journal Title: PLoS one Volume: 10
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Tetranorsesquiterpenoids and santalane-type sesquiterpenoids from Illicium lanceolatum and their antimicrobial activity against the oral pathogen Porphyromonas gingivalis. (2015)
  • Author(s): Kubo M, Nishikawa Y, Harada K, Oda M, Huang JM, Domon H, Terao Y, Fukuyama Y
  • Journal Title: J Nat Prod Volume: 78 Pages: 1466-1469
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Identification of the replication region in pBCNF5603, a bacteriocin-encoding plasmid, in the enterotoxigenic Clostridium perfringens strain F5603 (2015)
  • Author(s): Miyamoto K, Seike S, Takagishi T, Okui K, Oda M, Takehara M, Nagahama M
  • Journal Title: BMC Microbial Volume: 15 Pages: 118-118
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Total synthesis of palau’amine (2015)
  • Author(s): Namba K, Takeuchi K, Kaihara Y, Oda M, Nakayama A, Nakayama A, Yoshida M, Tanino K
  • Journal Title: Nat Commun Volume: 6 Pages: 8731-8731
  • NAID: 120006999407
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Membrane-binding mechanism of Clostridium perfringens alpha-toxin. (2015)
  • Author(s): Oda M, Terao Y, Sakurai J, Nagahama M
  • Journal Title: Toxins Volume: 7 Pages: 5268-5275
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Clostridium perfringens alpha-toxin induces GM1a clustering and TrkA phosphorylation in the host cell membrane. (2015)
  • Author(s): Takagishi, T., Oda, M., Kabura, M., Kurosawa, M., Tominaga, K., Urano, S., Ueda, Y.,Kobayashi, K., Kobayashi, T., Sakurai, J., Terao, Y., and Nagahama, M
  • Journal Title: PLoS one Volume: in press
  • Peer Reviewed
• [Journal Article] Endoplasmic reticulum stress response and bone loss in experimental periodontitis in mice. (2015)
  • Author(s): Yamada, H., Nakajima, T., Domon, H., Honda, T., and Yamazaki, K.
  • Journal Title: J. Periodontal Res. Volume: in press
  • Peer Reviewed
• [Journal Article] Residual structure of Streptococcus mutans biofilm following complete disinfection favors secondary bacterial adhesion and biofilm re-development. (2015)
  • Author(s): Ohsumi, T., Takenaka, S., Wakamatsu, R., Sakaue, Y., Narisawa, N., Senpuku, H., Ohshima, H., Terao, Y., and Okiji, T.:
  • Journal Title: PLoS one Volume: 10
  • Peer Reviewed
• [Journal Article] Vizantin inhibits endotoxin-mediated immune responses via the Toll-like receptor 4/MD-2 complex. (2014)
  • Author(s): Oda, M., Yamamoto, H., Shibutani, M., Nakano, M., Yabiku, K., Tarui, T., Kameyama, N., Shirakawa, D., Obayashi, S., Watanabe, N., Nakase, H., Suenaga, M., Matsunaga, Y., Nagahama, M., Takahashi, H., Imagawa, H., Kurosawa, M., Terao, Y., Nishizawa, M., and Sakurai, J.
  • Journal Title: J. Immunol. Volume: 193
  • Peer Reviewed
• [Journal Article] Age-related alterations in gene expression of gingival fibroblasts stimulated with Porphyromonas gingivalis. (2014)
  • Author(s): Domon, H., Nakajima, T., Tabeta, K., and Yamazaki, K.
  • Journal Title: J. Periodontal Res. Volume: 49 Pages: 536-543
  • Peer Reviewed
• [Presentation] Streptococcus pyogenesの咽頭上皮細胞株への付着・侵入におけるCAMP factorの役割 (2016)
  • Author(s): 黒澤美絵，小田真隆，土門久哲，寺尾 豊
  • Organizer: 第89回日本細菌学会総会
  • Place of Presentation: 大阪国際交流センター 大ホール（大阪府・大阪市）
  • Year and Date: 2016-03-23
• [Presentation] 肺炎球菌による肺組織傷害誘導メカニズムの解析 (2016)
  • Author(s): 土門久哲，坂上雄樹，小田真隆，山口雅也，川端重忠，寺尾 豊
  • Organizer: 第89回日本細菌学会総会
  • Place of Presentation: 大阪国際交流センター 大ホール（大阪府・大阪市）
  • Year and Date: 2016-03-23
• [Presentation] 多機能性糖脂質によるマクロファージ細胞外捕獲網形成機構の解明 (2016)
  • Author(s): 小田真隆，黒澤美絵，土門久哲，寺尾 豊
  • Organizer: 第89回日本細菌学会総会
  • Place of Presentation: 大阪国際交流センター 大ホール（大阪府・大阪市）
  • Year and Date: 2016-03-23
• [Presentation] Streptococcus pyogenesに起因する皮膚感染症の発症におけるSpeBの関与 (2015)
  • Author(s): 毛利 泰士，住友 倫子，山口 雅也，中田 匡宣，寺尾 豊，川端 重忠
  • Organizer: 第57回歯科基礎医学会学術大会
  • Place of Presentation: 朱鷺メッセ（新潟県・新潟市）
  • Year and Date: 2015-09-11
• [Presentation] Streptococcus pyogenes CAMP factorのRAW264.7細胞に対する空胞形成メカニズムの検討 (2015)
  • Author(s): 黒澤美絵，小田真隆，土門久哲，斎藤一誠，早崎治明，寺尾 豊
  • Organizer: 第57回歯科基礎医学会学術大会
  • Place of Presentation: 朱鷺メッセ（新潟県・新潟市）
  • Year and Date: 2015-09-11
• [Presentation] 肺炎球菌による宿主細胞の細胞死誘導能の解析 (2015)
  • Author(s): 土門久哲，坂上雄樹，小田真隆，山口雅也，川端重忠，寺尾 豊
  • Organizer: 第57回歯科基礎医学会学術大会
  • Place of Presentation: 朱鷺メッセ（新潟県・新潟市）
  • Year and Date: 2015-09-11
• [Presentation] 多機能性糖脂質によるマクロファージMETs形成メカニズムの解析 (2015)
  • Author(s): 小田真隆，黒澤美絵，土門久哲，寺尾 豊
  • Organizer: 第57回歯科基礎医学会学術大会
  • Place of Presentation: 朱鷺メッセ（新潟県・新潟市）
  • Year and Date: 2015-09-11
• [Presentation] Streptococcus pyogenes CAMP factorのRAW264.7細胞に対する病原性解析 (2015)
  • Author(s): 黒澤美絵，小田真隆，土門久哲，寺尾豊
  • Organizer: 第88回日本細菌学会総会
  • Place of Presentation: 岐阜，長良川国際会議場
  • Year and Date: 2015-03-25 – 2015-03-28
• [Presentation] Streptococcus mutansバイオフィルムに対する植物由来抽出物の検索 (2014)
  • Author(s): 坂上雄樹，土門久哲，小田真隆，興地隆史，寺尾豊
  • Organizer: 第51回日本細菌学会中部支部総会
  • Place of Presentation: 金沢，北陸大学薬学部
  • Year and Date: 2014-10-17 – 2014-10-18
• [Presentation] 慢性炎症性疾患におけるT L R シグナル抑制因子の解析 (2014)
  • Author(s): 黒澤美絵，小田真隆，土門久哲，寺尾 豊
  • Organizer: 第51回日本細菌学会中部支部総会
  • Place of Presentation: 金沢，北陸大学薬学部
  • Year and Date: 2014-10-17 – 2014-10-18
• [Presentation] 多機能性糖脂質の自然免疫活性化メカニズムの解明 (2014)
  • Author(s): 小田真隆，山本博文，黒澤美絵，土門久哲，寺尾 豊
  • Organizer: 第51回日本細菌学会中部支部総会
  • Place of Presentation: 金沢，北陸大学薬学部
  • Year and Date: 2014-10-17 – 2014-10-18
• [Presentation] イメージングと細菌学研究 (2014)
  • Author(s): 寺尾 豊
  • Organizer: 第67回 日本細菌学会中国・四国支部総会
  • Place of Presentation: 徳島，徳島文理大学薬学部
  • Year and Date: 2014-10-04
  • Invited
• [Presentation] A群レンサ球菌CAMPファクターのマクロファージに対する病原性解析 (2014)
  • Author(s): 小田真隆，黒澤美絵，土門久哲，寺尾豊
  • Organizer: 第56回歯科基礎医学会学術大会・総会
  • Place of Presentation: 福岡，福岡国際会議場
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 歯周炎におけるTLR シグナル抑制因子の解析 (2014)
  • Author(s): 土門久哲，小田真隆，寺尾豊
  • Organizer: 第56回歯科基礎医学会学術大会・総会
  • Place of Presentation: 福岡，福岡国際会議場
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 合成糖脂質のStreptococcus mutansバイオフィルムに対する作用 (2014)
  • Author(s): 黒澤美絵，小田真隆，土門久哲，寺尾豊
  • Organizer: 第56回歯科基礎医学会学術大会・総会
  • Place of Presentation: 福岡，福岡国際会議場
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Streptococcus mutansに対する植物由来抽出物の殺菌ならびに抗バイオフィルム効果の検討 (2014)
  • Author(s): 坂上雄樹，土門久哲，小田真隆，興地隆史，寺尾豊
  • Organizer: 第56回歯科基礎医学会学術大会・総会
  • Place of Presentation: 福岡，福岡国際会議場
  • Year and Date: 2014-09-25 – 2014-09-27
• [Book] 口腔微生物学・免疫学 第４版 (2015)
  • Author(s): 川端重忠，小松澤均，大原直也，寺尾 豊，浜田茂幸編
  • Total Pages: 303
  • Publisher: 医歯薬出版
• [Book] 日本歯科評論：ナノスケールの研究を歯科医学に， (2014)
  • Author(s): 寺尾 豊
  • Total Pages: 181
  • Publisher: ヒョーロン・パブリッシャーズ社
• [Remarks] 新潟大学 大学院医歯学総合研究科 微生物感染症学分野
  • URL: http://www.dent.niigata-u.ac.jp/microbio/microbio.html
• [Remarks] 新潟大学 大学院医歯学総合研究科 微生物感染症学分野 研究内容
  • URL: http://www.niigata-univ-mid.net/study/index4.html
• [Remarks] 研究者の作成HP
  • URL: http://www.dent.niigata-u.ac.jp/microbio/microbio.html