| Project/Area Number |
26670914
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Fundamental nursing
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MINEMATSU Takeo 東京大学, 医学(系)研究科(研究院), 講師 (00398752)
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| Co-Investigator(Kenkyū-buntansha) |
SANADA Hiromi 東京大学, 大学院医学系研究科, 教授 (50143920)
MORI Taketoshi 東京大学, 大学院医学系研究科, 教授 (20272586)
NAKAGAMI Gojiro 東京大学, 大学院医学系研究科, 講師 (70547827)
NOGUCHI Hiroshi 東京大学, 大学院医学系研究科, 講師 (50431797)
TAMAI Nao 東京大学, 大学院医学系研究科, 助教 (80636788)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | スキンアセスメント / 褥瘡 / スキンケア / 看護技術 / スキンブロッティング / 皮膚バリア機能 / 角質細胞間脂質 / タイトジャンクション |
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| Outline of Final Research Achievements |
(1)The principle of skin blotting: We could not fully reveal the principle of skin blotting within this period. Conventional techniques are not enough for the analysis of the effect of skin blotting on the inter-corneocyte lipid lamellae structure. Therefore, we developed new fixation technique of skin tissue, which can keep the structures of inter-corneocyte lipid, epidermis and dermis. Furthermore, the temporal structural change by skin blotting was suggested by immunofluorescence for claudin-1, a tight junction protein.
(2)In the animal experiment, we identified the biomarkers for early detection of pressure ulcer development by skin blotting. The biomarkers are PAI1, VEGF-C, IL1a, and HSP90a, which reflected the ischemia, lymphatic dysfunction, ischemia-reperfusion injury, and deformation of tissue, respectively.
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