Combined approach of In vitro and in vivo analysis for mechanisms of cytokinesis in fission yeast
| Project/Area Number |
26712010
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | Kobe University (2016-2018)
Gakushuin University (2014-2015) |
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Principal Investigator |
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| Research Collaborator |
MABUCHI Issei
TAKATSU Masako
TAKAGI Tomoko
AMIKURA Reiko
ARAI Ritsuko
UEDA Ei-ichi
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| Project Period (FY) |
2014-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2017: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
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| Keywords | 細胞質分裂 / 収縮環 / 分裂酵母 / ミオシン / アクチン / 細胞分裂 / 細胞骨格 / 細胞・組織 / 菌類 / 遺伝学 |
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| Outline of Final Research Achievements |
We study the mechanisms for cytokinesis, which is the dynamic process accompanied by the whole cell deformation using fission yeast as a model organism. It has been found that actin polymerization and depolymerization is important at each step in cytokinesis. To elucidate the mechanism for contraction of the contractile ring, I constructed the tool to analyze distribution and orientation of myosin II in a contractile ring by ultrastructural analyses and super resolution microscopies using the cell ghosts or the isolated rings. In addition, we found the special condition for cleavage furrow ingression in the fission yeast spheroplasts in the presence of cell wall-degradative enzymes.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究の成果により、細胞質分裂、細胞骨格研究分野に新たな知見を与えることができ、今後もさらなる成果が期待できる。細胞分裂は単細胞生物ではその増殖に、多細胞生物では発生、成長、個体の維持に必須の生命現象である。細胞質分裂がうまくいかないと細胞は多核になり、がん化する原因となり得る。細胞質分裂、収縮環の形成や収縮の仕組みを深く知ることは、医学・薬学分野にも有用な知見となる。また、病原性の真菌が多く知られているが、その治療や感染予防にも応用できる可能性がある。
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Report
(6 results)
Research Products
(18 results)
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[Presentation] Sculpting the ring to make a cut: contractile ring structure and mechanism of cell division2017
Author(s)
Mishra, M., Swulius, M.T., Nguyen., L.T., Aiech, S., Kashiwazaki, J., Srinivasan, R., Balasubramanian, M.K., Mabuchi, I., Jensen, G
Organizer
第69回日本細胞生物学会大会
Related Report
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