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Analysis of intracellular pathogenic pathway of Legionella pneumophila

Research Project

Project/Area Number 26713016
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Bacteriology (including mycology)
Research InstitutionTokyo University of Pharmacy and Life Science

Principal Investigator

Arasaki Kohei  東京薬科大学, 生命科学部, 講師 (70609990)

Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Keywordsレジオネラ / レジオネラエフェクター / Rabタンパク質 / 小胞輸送 / 小胞体 / 膜輸送 / ユビキチン / 小胞体サブドメイン / Bap31 / リソソーム / エフェクター / 細胞内小胞輸送 / プロテアソーム
Outline of Final Research Achievements

In this research, we demonstrated following three things. 1: Legionella manipulates Rab33b-Rab6 cascade to escort LCV to the host ER and we identified the candidate effectors that recruits Rab33b to the Legionella containing vacuole (LCV). 2: After internalization into host cells, Legionella blocks delivery of LCV to the lysosomal compartment. In this process, Legionella degrades Rab5 by ubiquitin dependent manner and we identified E3 ligase like effector against Rab5. 3: It is known that Legionella replicates inside host ER. In this process, we demonstrated that Legionella first associates and fuses with smooth ER to evade host ER. Subsequently, Legionella moves to rough ER and starts to replicates. In this traffic, we identified the host protein that requires traffic of Legionella from the smooth to the rough ER. Furthermore, we also identified the candidate effector that subverts this host factor.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (31 results)

All 2018 2017 2016 2015 2014

All Journal Article (10 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 9 results,  Open Access: 5 results,  Acknowledgement Compliant: 2 results) Presentation (21 results) (of which Invited: 7 results)

  • [Journal Article] Syntaxin 17 promotes lipid droplet formation by regulating the distribution of acyl-CoA synthetase 3.2018

    • Author(s)
      Kimura H, Arasaki K, Ohsaki Y, Fujimoto T, Ohtomo T, Yamada J, Tagaya M.
    • Journal Title

      J Lipid Res.

      Volume: in press Issue: 5 Pages: 805-819

    • DOI

      10.1194/jlr.m081679

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Legionella blocks autophagy by cleaving STX17 (syntaxin 17).2017

    • Author(s)
      Arasaki K, Tagaya M.
    • Journal Title

      Autophagy.

      Volume: 13 Issue: 11 Pages: 2008-2009

    • DOI

      10.1080/15548627.2017.1371395

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Legionella effector Lpg1137 shuts down ER-mitochondria communication through cleavage of syntaxin 17.2017

    • Author(s)
      Arasaki K, Mikami Y, Shames SR, Inoue H, Wakana Y, Tagaya M.
    • Journal Title

      Nat Commun.

      Volume: 15406 Issue: 1 Pages: 1-12

    • DOI

      10.1038/ncomms15406

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Regulation of Mitochondrial Dynamics and Autophagy by the Mitochondria-Associated Membrane.2017

    • Author(s)
      Tagaya M, Arasaki K.
    • Journal Title

      Adv Exp Med Biol.

      Volume: 997 Pages: 33-47

    • DOI

      10.1007/978-981-10-4567-7_3

    • ISBN
      9789811045660, 9789811045677
    • Related Report
      2017 Annual Research Report
  • [Journal Article] Legionella Effector Lpg1137 Shuts down ER-mitochondria Communication through Cleavage of Syntaxin 172017

    • Author(s)
      Arasaki K., Mikami Y., Shames SR., Inoue H., Wakana Y., Tagaya M.
    • Journal Title

      Nature Communications

      Volume: in press

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] γ-SNAP stimulates disassembly of endosomal SNARE complexes and regulates endocytic trafficking pathways.2015

    • Author(s)
      Inoue, H., Matsuzaki, Y., Tanaka, A., Hosoi, K., Ichimura, K., Arasaki, K., Wakana, Y., Asano, K., Tanaka, M., Okuzaki, D., Yamamoto, A., Tani, K. and Tagaya, M.
    • Journal Title

      J. Cell Sci.

      Volume: 128 Pages: 2781-2794

    • DOI

      10.1242/jcs.158634

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] CARTS biogenesis requires VAP-lipid transfer protein complexes functioning at the endoplasmic reticulum-Golgi interface.2015

    • Author(s)
      Wakana Y., Kotake R., Oyama N., Murate M., Kobayashi T., Arasaki K., Inoue H., Tagaya M.
    • Journal Title

      Mol. Biol Cell

      Volume: 26 Issue: 25 Pages: 4686-4699

    • DOI

      10.1091/mbc.e15-08-0599

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Role for the ancient SNARE syntaxin 17 in regulating mitochondrial division.2015

    • Author(s)
      Arasaki, K., Shimizu, H., Mogari, H., Nishida, N., Hirota, N., Furuno, A., Kudo, Y., Baba, M., Baba, N., Cheng, J., Fujimoto, T., Ishihara, N., Ortiz-Sandoval, C., Barlow, L. D., Raturi, A., Dohmae, N., Wakana, Y., Inoue, H., Tani, K., Dacks, J. B., Simmen, T., and Tagaya, M.
    • Journal Title

      Developmental Cell

      Volume: 32 Pages: 304-317

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The machinery at endoplasmic reticulum-plasma membrane contact sites contributes to spatial regulation of multiple Legionella effector proteins.2014

    • Author(s)
      Hubber, A., Arasaki, K., Nakatsu, F., Hardiman, C.,Lambright, D., De Camilli, P., Nagai, H., and Roy, C. R.
    • Journal Title

      PLoS Pathogens

      Volume: 10

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Valosin-containing protein-interacting membrane protein (VIMP) links the endoplasmic reticulum with microtubules in concert with cytoskeleton-linking membrane protein (CLIMP)-63.2014

    • Author(s)
      Noda, C*., Kimura, H*., Arasaki, K*., Matsushita, M., Yamamoto, A., Wakana, Y., Inoue, H., and Tagaya, M.
    • Journal Title

      Journal of Biologicalchemistery

      Volume: 289 Pages: 24304-24313

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] Syntaxin 17はACSL3の局在と機能を制御することで脂肪滴形成を促進する2018

    • Author(s)
      新崎恒平・木村葉那・大崎雄樹・多賀谷光男
    • Organizer
      第123回 日本解剖学会総会 シンポジウム
    • Related Report
      2017 Annual Research Report
  • [Presentation] Legionella Effector Lpg1137 Shuts down ER-mitochondria Communication through Cleavage of Syntaxin 172017

    • Author(s)
      新崎恒平、三上優美、James Havey、Stephanie Shames、Craig Roy、多賀谷光男
    • Organizer
      第90回 日本細菌学会総会
    • Place of Presentation
      仙台
    • Year and Date
      2017-03-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] レジオネラはsyntaxin 17の分解を介して宿主防御機構を回避する2017

    • Author(s)
      新崎恒平
    • Organizer
      第100回 日本細菌学会関東支部総会 シンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 小胞体-ミトコンドリア接触部位におけるsyntaxin 17の多彩な生理機能2017

    • Author(s)
      新崎恒平、多賀谷光男
    • Organizer
      第90回 日本生化学会大会 ワークショップ
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 小胞体SNAREタンパク質の多様な機能2017

    • Author(s)
      新崎恒平
    • Organizer
      第90回 日本生化学会大会 受賞記念講演
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] MAP1B-LC1による脂肪滴形成の制御2017

    • Author(s)
      木村葉那、新崎恒平、大崎雄樹、多賀谷光男
    • Organizer
      第90回 日本生化学会大会 ポスター発表
    • Related Report
      2017 Annual Research Report
  • [Presentation] Syntaxin 17 promotes lipiddroplet formation by regulating the distribution of ACSL3, a key enzyme for lipid droplet biogenesis.2016

    • Author(s)
      Hana Kimura, Kohei Arasaki, Mitsuo Tagaya
    • Organizer
      2016 American Society of Cell Biology
    • Place of Presentation
      San francisco
    • Year and Date
      2016-12-03
    • Related Report
      2016 Annual Research Report
  • [Presentation] MAP1B-LC1 regulates autophagosome formation by interacting with the autophagy-related SNARE syntaxin 172016

    • Author(s)
      Kohei Arasaki, Mitsuo Tagaya, Naoshi Dohmae, Yuri Kurosawa, Haruki Nagashima
    • Organizer
      2016 American Society of Cell Biology
    • Place of Presentation
      San francisco
    • Year and Date
      2016-12-03
    • Related Report
      2016 Annual Research Report
  • [Presentation] The autophagic function of syntaxin 17 is suppressed by MAP1B-LC12016

    • Author(s)
      新崎恒平、 長島晴輝、 黒澤優里、 堂前直、多賀谷光男
    • Organizer
      第89回 日本生化学会大会
    • Place of Presentation
      仙台
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Syntaxin 17 regulates the lipid droplets localization of ACSL3 through interaction with SNAP-232016

    • Author(s)
      木村葉那、 新崎恒平、 多賀谷光男
    • Organizer
      第89回 日本生化学会大会
    • Place of Presentation
      仙台
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] 脂肪滴形成におけるMAMの役割~Stx17の機能を中心として~2016

    • Author(s)
      新崎 恒平、木村 葉那、大崎 雄樹、多賀谷 光男
    • Organizer
      第121回 日本解剖学会総会
    • Place of Presentation
      ビックパレットふくしま
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Stx17はACSL3を制御することで脂肪滴形成に関与する2016

    • Author(s)
      木村 葉那、新崎 恒平、大崎 雄樹、多賀谷 光男
    • Organizer
      第121回 日本解剖学会総会
    • Place of Presentation
      ビックパレットふくしま
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] Rab6A and Rab33B function in Legionella infection2016

    • Author(s)
      川端 美緒、多賀谷 光男、新崎 恒平
    • Organizer
      第89回 日本細菌学会総会
    • Place of Presentation
      大阪国際交流センター
    • Year and Date
      2016-03-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] 脂肪滴の生合成形におけるsyntaxin 17の関与2015

    • Author(s)
      木村 葉那、新崎 恒平、多賀谷 光男
    • Organizer
      第88回 日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] MAP1B-LC1はsyntaxin17との相互作用を介してオートファジーを制御する2015

    • Author(s)
      長島 晴輝、新崎 恒平、黒澤 優里、堂前 直、多賀谷 光男
    • Organizer
      第88回 日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] レジオネラ菌感染に関わるRabタンパク質の同定及びレジオネラ菌の小胞体定着化機構2015

    • Author(s)
      新崎恒平、川端美緒、加藤郁子、多賀谷光男
    • Organizer
      第88回日本細菌学会総会
    • Place of Presentation
      岐阜
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] レジオネラ菌によるRab5ユビキチン化機構の解明2015

    • Author(s)
      川端美緒、加藤郁子、多賀谷光男、新崎恒平
    • Organizer
      第88回日本細菌学会総会
    • Place of Presentation
      岐阜
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] レジオネラ菌は小胞体内での移行にRab4及びRab10を利用する2015

    • Author(s)
      加藤郁子、川端美緒、新崎恒平、多賀谷光男
    • Organizer
      第88回日本細菌学会総会
    • Place of Presentation
      岐阜
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] レジオネラ菌の宿主小胞体への移行及び定着化の機構2014

    • Author(s)
      新崎恒平、川端美緒、野内優、多賀谷光男
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] PGAM5によるDrp1の脱リン酸化へのSyntaxin17の関与2014

    • Author(s)
      須合真士、新崎恒平、堂前直、多賀谷光男
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 脂肪滴形成および3T3-L1の脂肪細胞への分化におけるsyntaxin 17の関与2014

    • Author(s)
      木村葉那、新崎恒平、茂刈寛史、谷佳津子、多賀谷光男
    • Organizer
      第66回日本細胞生物学会大会
    • Place of Presentation
      奈良
    • Year and Date
      2014-06-11 – 2014-06-13
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2019-03-29  

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