| Project/Area Number |
26790035
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nano/Microsystems
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Seichepine Florent 国立研究開発法人理化学研究所, 生命システムセンター, 特別研究員 (20650641)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | Neurotransmitter / CMOS / Microelectrode Array / Carbon nanotube / sensors / Sensors / Ｃａｒｂｏｎ ｎａｎｏｔｕｂｅ / biosensors / neurotransmitters / biofunctionalization / Nanosensors |
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| Outline of Final Research Achievements |
We have created a high-density array of carbon nanotube field effect transistor sensors (CNT-FET) and we have proved that they can be used for the sensing of neurotransmitters after functionalization with specific enzyme. We have demonstrated the possibility of developing culture of neurons on those sensors and we have successfully records under those conditions. More specifically, we have created new type of CNT-FET sensors using electric field manipulation with yield of about 80% of small bundle on 1024 devices. By using liquid gate configuration, we have proved the high-sensitivity of our sensors. We have use non-covalent functionalization methods to bind glutamate oxidase to CNT-FET and we have shown that such biosensors are sensitive to glutamate. This accomplishment represents the fabrication of a new tool to investigate chemical release in culture medium which can be co-integrated with electrical recording.
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