| Project/Area Number |
26810091
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Yamaguchi Takao 東京大学, 分子細胞生物学研究所, 助教 (80596601)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | アフィニティーラベル化 / 核酸アプタマー / タンパク質化学修飾 / 蛍光ラベル化 |
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| Outline of Final Research Achievements |
Chemical probes, consist of an oligonucleotide aptamer and a fluorogenic reactive group, were designed to achieve a specific labeling of protein of interest (POI). Here, we prepared oligonucleotide aptamer for c-Met, a model POI. In addition, several fluorogenic reactive groups were designed and synthesized. Model experiments revealed that one of the designed fluorogenic reactive groups has a suitable stability and reactivity for the POI-specific labeling.
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