Gene expression analysis along the tonotopic axis of the chicken

Research Project

Project/Area Number	26830011
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Neurophysiology / General neuroscience
Research Institution	Kyoto University
Principal Investigator	Matsui Ryosuke 京都大学, 医学研究科, 助教 (80633940)
Research Collaborator	WATANABE DAI 京都大学, 医学研究科
Project Period (FY)	2014-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords	音源定位 / トノトピー / 遺伝子発現 / 分子プロファイリング / 層状核 / 大細胞核 / 神経回路形成 / 形態形成
Outline of Final Research Achievements	Neurons in the auditory pathway are topographically arranged by their response to different auditory frequencies. Although tonotopic organization is a fundamental structure for frequency processing, the molecular mechanisms of the tonotopic map formation are largely unknown. In this study, we analyzed the gene expression profiles of two brainstem regions, nucleus laminaris and nucleus magnocellularis, to identify genes that are differentially expressed along the tonotopic axis. As a result, more than hundred molecules expressed in frequency-dependent manner and the most enriched pathway was axon guidance. These data should be useful for understanding the molecular mechanisms of the tonotopic map formation.