Analysis of nuclear and cytoskeletal dynamics underlying neuronal migration

• Project/Area Number: 26830012
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology / General neuroscience
• Research Institution: Kyoto University
• Principal Investigator: Umeshima Hiroki 京都大学, 物質－細胞統合システム拠点, 研究員 (40525375)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 神経発生 / 細胞移動 / 脳・神経 / 発生・分化 / 細胞・組織

Outline of Final Research Achievements

I performed time-lapse imaging of nuclear translocation during neuronal migration with a high-speed confocal microscope, in order to understand molecular mechanisms of neuronal migration. As a result, I found that the nucleus is deformed and rotated during neuronal migration. Moreover, molecular biological approaches suggested that force generated by microbule-based motor proteins drives both nuclear translocation and rotation of migrating neurons (submitting a manuscript for publication). On the other hand, I also performed traction force microscope analysis for migrating neurons and found traction force accompanied by nuclear translocation, which is generated by actomyosin contractile activity (preparing a manucscript).

Research Products

• [Journal Article] ECHO-liveFISH: in vivo RNA labeling reveals dynamic regulation of nuclear RNA foci in living tissues. (2015)
  • Author(s): Oomoto, I., Suzuki-Hirano, A., Umeshima, H., Han, YW., Yanagisawa, H., Carlton, P., Harada, Y., Kengaku, M., Okamoto, A., Shimogori, T. and Wang, DO.
  • Journal Title: Nucleic Acids Res. Volume: 43(19) Issue: 19 Pages: e126-e126
  • DOI: 10.1093/nar/gkv614
  • NAID: 120005619785
  • Peer Reviewed / Open Access
• [Journal Article] Cerebellar granule cells are predominantly generated by terminal symmetric divisions of granule cell precursors. (2015)
  • Author(s): Nakashima, K., Umeshima, H. and Kengaku M.
  • Journal Title: Dev. Dyn. Volume: 244(6) Issue: 6 Pages: 748-758
  • DOI: 10.1002/dvdy.24276
  • Peer Reviewed
• [Presentation] Cell mechanics underlying nuclear translocation of migrationg neurons. (2015)
  • Author(s): Hiroki Umeshima, You Kure Wu, Kenichi Nomura, Shuhei Yoshikawa, Shinya Sakuma, Fumihito Arai, Makoto Kaneko and Mineko Kengaku
  • Organizer: Neurosience2015
  • Place of Presentation: Chicago, USA
  • Year and Date: 2015-10-17
  • Int'l Joint Research
• [Presentation] Cell mechanics underlying nuclear translocation of migrationg neurons. (2015)
  • Author(s): Hiroki Umeshima, Yuu Kure, Kenichi Nomura, Shuhei Yoshikawa, Shinya Sakuma, Fumihito Arai, Makoto Kaneko, Mineko Kengaku
  • Organizer: iCeMS International Symposium ‘Hierarchical Dynamics in Soft Materials and Biological Matter’
  • Place of Presentation: 京都大学北部総合教育研究棟益川ホール（京都府・京都市）
  • Year and Date: 2015-09-23
  • Int'l Joint Research
• [Presentation] Cell mechanics underlying nuclear translocation of migrating neurons. (2015)
  • Author(s): Hiroki Umeshima, You Kure Woo, Kenichi Nomura, Shuhei Yoshikawa, Shinya Sakuma, Fumihito Arai, Makoto Kaneko, Mineko Kengaku
  • Organizer: The 14th International Joint Mini-Symposium on Molecular and Cell Biology between KU-NTU-UT
  • Place of Presentation: 京都大学物質－細胞統合システム拠点本館２階セミナー室（京都府・京都市）
  • Year and Date: 2015-06-13
  • Int'l Joint Research
• [Presentation] Quantitative Image Analyses of Nuclear Dynamics in Migrating Neurons (2014)
  • Author(s): Hiroki Umeshima, Yuu Kure, Kenichi Nomura, Shuhei Yoshikawa, Shinya Sakuma, Fumihito Arai, Makoto Kaneko and Mineko Kengaku
  • Organizer: 2014 International Symposium on Micro-NanoMechatronics and Human Science
  • Place of Presentation: 名古屋大学（名古屋市）
  • Year and Date: 2014-11-11
• [Presentation] Mechanical approaches to nuclear migration of neurons during brain formation (2014)
  • Author(s): Hiroki Umeshima
  • Organizer: 文部科学省科学研究費補助金新学術領域「超高速バイオアセンブラ」 京都大学・物質-細胞統合システム拠点 合同シンポジウム
  • Place of Presentation: 京都大学（京都市）
  • Year and Date: 2014-05-16
  • Invited