| Project/Area Number |
26840055
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Yanagawa Masataka 国立研究開発法人理化学研究所, 佐甲細胞情報研究室, 研究員 (70609792)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | GPCR / 代謝型グルタミン酸受容体 / セロトニン受容体 / 1分子イメージング / 多量体化 / FRET / 生物物理学 / 薬理学 / 生化学 / 統合失調症 |
|---|
| Outline of Final Research Achievements |
G protein-coupled receptors (GPCRs) are major drug targets. It has been reported that various GPCRs form dimers regulating their functions. However, a role of higher-order oligomerization of GPCRs in living cell is unclear. Here, we performed single-molecule imaging and FRET analysis of metabotropic glutamate receptor (mGluR) and serotonine receptor (5HTR) as model GPCRs. Single-molecule imaging analysis in living cell demonstrated that the fraction of the immobile higher-order oligomer of mGluRs, which is related to the recruitment of receptors into clathrin-coated pits, increased upon activation. The FRET analysis suggested that a dissociation of mGluR/5HTR hetero-oligomer occurs upon activation of mGluR.
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