Comprehensive study on chloroplast redox regulation network

• Project/Area Number: 26840090
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Yoshida Keisuke 東京工業大学, 資源化学研究所, 助教 (40632310)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: レドックス制御 / チオレドキシン / 葉緑体 / 光合成 / シロイヌナズナ

Outline of Final Research Achievements

Plant chloroplast has a redox-based regulatory network for adjusting its biological systems to environmental fluctuations. In this study, I performed a comprehensive study addressing the molecular basis and physiological significance of chloroplast redox regulation network. The results showed that this system is highly organized beyond current expectation. I further clarified that this system drastically responds to environmental fluctuations and plays an important role in plant biomass production.

Research Products

• [Journal Article] Oxidation of a cysteine residue in elongation factor EF-Tu reversibly inhibits translation in the cyanobacterium Synechocystis sp. PCC 6803 (2016)
  • Author(s): Yutthanasirikul, R., Nagano, T., Jimbo, H., Hihara, Y., Kanamori, T., Ueda, T., Haruyama, T., Konno, H., Yoshida, K., Hisabori, T. and Nishiyama, Y.
  • Journal Title: J. Biol. Chem. Volume: 291(11) Issue: 11 Pages: 5860-5870
  • DOI: 10.1074/jbc.m115.706424
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Adenine nucleotide-dependent and redox-independent control of mitochondrial malate dehydrogenase activity in Arabidopsis thaliana. (2016)
  • Author(s): Yoshida K., Hisabori T.
  • Journal Title: Biochimica et Biophysica Acta Volume: in press Issue: 6 Pages: 810-818
  • DOI: 10.1016/j.bbabio.2016.03.001
  • Peer Reviewed / Open Access
• [Journal Article] Thioredoxin selectivity for thiol-based redox regulation of target proteins in chloroplasts (2015)
  • Author(s): Yoshida K., Hara S., Hisabori T.
  • Journal Title: Journal of Biological Chemistry Volume: in press Issue: 23 Pages: 14278-14288
  • DOI: 10.1074/jbc.m115.647545
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Distinct redox behaviors of chloroplast thiol enzymes and their relationships with photosynthetic electron transport in Arabidopsis thaliana.. (2014)
  • Author(s): Yoshida K, Matsuoka Y, Hara S, Konno H, Hisabori T.
  • Journal Title: Plant Cell Physiol. Volume: 55 Issue: 8 Pages: 1415-1425
  • DOI: 10.1093/pcp/pcu066
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Mitochondrial isocitrate dehydrogenase is inactivated upon oxidation and reactivated by thioredoxin-dependent reduction in Arabidopsis (2014)
  • Author(s): Yoshida K., Hisabori T.
  • Journal Title: Frontiers in Environmental Science Volume: 2 Pages: 38-38
  • DOI: 10.3389/fenvs.2014.00038
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 葉緑体レドックスネットワークにおける還元力伝達の複雑さ：シロイヌナズナFTRヘテロ二量体は10種のTrxを異なる効率で還元する (2016)
  • Author(s): 吉田啓亮、久堀徹
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 盛岡
  • Year and Date: 2016-03-18
• [Presentation] Anabaena sp. PCC 7120のNADPH-thioredoxin reductase Cは抗酸化ストレス系に重要である (2016)
  • Author(s): 見原翔子、吉田啓亮、肥後明佳、久堀徹
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 盛岡
  • Year and Date: 2016-03-18
• [Presentation] Significance of the redox regulation networks in plants (2015)
  • Author(s): Keisuke Yoshida, Satoshi Hara, Kazunori Sugiura, Toru Hisabori
  • Organizer: Yamada Conference International Symposium on Dynamics and Regulation of Photosynthesis
  • Place of Presentation: Nara
  • Year and Date: 2015-10-29
  • Invited
• [Presentation] 葉緑体レドックスネットワークによる光合成の機能制御 (2015)
  • Author(s): 吉田啓亮
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 新潟
  • Year and Date: 2015-09-06
  • Invited
• [Presentation] ミトコンドリアTCAサイクル酵素のレドックスとアデニレート状態に応じた新奇活性調節メカニズム (2015)
  • Author(s): 吉田啓亮、久堀徹
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 新潟
  • Year and Date: 2015-09-06
• [Presentation] FTR/Trx経路とNTRC経路は協調的に葉緑体機能のレドックス制御および植物の生育を支えている (2015)
  • Author(s): 吉田啓亮、久堀徹
  • Organizer: 第6回日本光合成学会
  • Place of Presentation: 岡山
  • Year and Date: 2015-05-22
• [Presentation] Anabaena sp. PCC 7120におけるNADPH-Thioredoxin Reductase Cの機能解析 (2015)
  • Author(s): 見原翔子、吉田啓亮、肥後明佳、久堀徹
  • Organizer: 第6回日本光合成学会
  • Place of Presentation: 岡山
  • Year and Date: 2015-05-22
• [Presentation] シロイヌナズナ変異株解析から見えてきた葉緑体の機能調節におけるレドックス制御の重要性 (2015)
  • Author(s): 吉田啓亮、久堀徹
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学世田谷キャンパス
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Anabaena sp. PCC 7120におけるNTRCの標的タンパク質の探索 (2015)
  • Author(s): 見原翔子、吉田啓亮、肥後明佳、久堀徹
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京農業大学世田谷キャンパス
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 植物体内のレドックス制御を可視化する (2014)
  • Author(s): 久堀徹、杉浦一徳、原怜、永井健治、吉田啓亮
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2014-10-15 – 2014-10-18
  • Invited
• [Presentation] 光合成生物細胞内のレドックス環境の可視化 (2014)
  • Author(s): 杉浦一徳、永井健治、原怜、吉田啓亮、久堀徹
  • Organizer: 日本遺伝学会第86回大会
  • Place of Presentation: 長浜バイオ大学
  • Year and Date: 2014-09-17 – 2014-09-19
  • Invited
• [Presentation] 葉緑体リンゴ酸脱水素酵素のレドックス制御のチオレドキシン特異性：Trx-mよりTrx-fが重要である (2014)
  • Author(s): 吉田啓亮、原怜、久堀徹
  • Organizer: 日本植物学会第78回大会
  • Place of Presentation: 明治大学生田キャンパス
  • Year and Date: 2014-09-12 – 2014-09-14
• [Book] 葉緑体とミトコンドリアの代謝クロストーク (2015)
  • Author(s): 吉田啓亮
  • Total Pages: 9
  • Publisher: 日本生物科学者協会
• [Remarks] Hisabori-Wakabayashi Laboratory
  • URL: http://www.res.titech.ac.jp/~junkan/Hisabori_HomePage/index.html