How the plant body axis is to established under the transcriptional network that is activated by fertilization?

• Project/Area Number: 26840093
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nagoya University
• Principal Investigator: Ueda Minako 名古屋大学, 理学研究科(WPI), 特任講師 (20598726)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 体軸形成 / 細胞極性 / 植物発生

Outline of Final Research Achievements

In many plants, the asymmetric division of the zygote sets up the apical-basal axis of the embryo. We have established the live-imaging method to visualize how the zygote polarizes after fertilization, and provided insights into the intracellular dynamics of zygote polarization in flowering plants (Kimata et al, 2016). In addition, we have revealed a framework of how maternal and paternal factors are integrated in the zygote to regulate embryo patterning (Ueda et al, 2017).

Research Products

• [Journal Article] Transcriptional integration of paternal and maternal factors in the Arabidopsis zygote (2017)
  • Author(s): Ueda M, Aichinger E, Gong W, Groot E, Verstraeten I, Vu LD, De Smet I, Higashiyama T, Umeda M, Laux T
  • Journal Title: Genes & Development Volume: 31 Issue: 6 Pages: 617-627
  • DOI: 10.1101/gad.292409.116
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Cytoskeleton dynamics control the first asymmetric cell division in Arabidopsis zygote. (2016)
  • Author(s): Kimata, Y., Higaki, T., Kawashima, T., Kurihara, D., Sato, Y., Yamada, T., Hasezawa, S., Berger, F., Higashiyama, T., and Ueda, M.
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 113 Issue: 49 Pages: 14157-14162
  • DOI: 10.1073/pnas.1613979113
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Rapid Elimination of the Persistent Synergid through a Cell Fusion Mechanism. (2015)
  • Author(s): 丸山大輔、Ronny Völz、竹内英憲、森稔幸、井川智子、栗原大輔、河島友和、植田美那子、伊藤正樹、梅田正明、西川周一、Rita Groß-Hardt、東山哲也
  • Journal Title: Cell Volume: 未定 Issue: 4 Pages: 907-918
  • DOI: 10.1016/j.cell.2015.03.018
  • Peer Reviewed / Open Access
• [Journal Article] Live-Cell Imaging and Optical Manipulation of Arabidopsis Early Embryogenesis (2015)
  • Author(s): Keita Gooh, Minako Ueda, Kana Aruga, Jongho Park, Hideyuki Arata, Tetsuya Higashiyama, and Daisuke Kurihara
  • Journal Title: Developmental Cell Volume: 34 Issue: 2 Pages: 242-251
  • DOI: 10.1016/j.devcel.2015.06.008
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 植物の受精卵はどのように極性化するのか？ (2017)
  • Author(s): 植田美那子，木全祐資，栗原大輔，山田朋美，東山哲也
  • Organizer: 第58会植物生理学会年会
  • Place of Presentation: 鹿児島大学郡元キャンパス
  • Year and Date: 2017-03-16
• [Presentation] 植物受精卵のライブイメージング： 細胞極性とは何か? (2016)
  • Author(s): 木全祐資, 栗原大輔, 桧垣匠, 河島友和, 佐藤良勝, 山田朋美, Frederic Berger, 馳澤盛一郎, 東山哲也, 植田美那子
  • Organizer: 日本植物学会第80回大会
  • Place of Presentation: 沖縄コンベンションセンター
  • Year and Date: 2016-09-16
• [Presentation] Live-cell imaging of the intracellular dynamics underlying the zygote polarization in Arabidopsis (2016)
  • Author(s): Yusuke Kimata, Daisuke Kurihara, Takumi Higaki, Tomomi Yamada, Seiichiro Hasezawa, Tetsuya Higashiyama and Minako Ueda
  • Organizer: 27th International Conference on Arabidopsis Research (ICAR2016)
  • Place of Presentation: 慶州 (韓国)
  • Year and Date: 2016-06-29
  • Int'l Joint Research / Invited
• [Presentation] Live imaging of the intracellular dynamics during the zygote polarization in Arabidopsis (2016)
  • Author(s): Yusuke Kimata, Daisuke Kurihara, Ayato Sato, Tetsuya Higashiyama, Minako Ueda
  • Organizer: The 24th IASPRR conference
  • Place of Presentation: Tucson, Arizona (USA)
  • Year and Date: 2016-03-18
  • Int'l Joint Research
• [Presentation] Dynamics of zygote polarization: How plant forms the apical-basal axis 受精卵の極性化動態 ～植物の体軸形成のしくみ～ (2015)
  • Author(s): 木全祐資，栗原大輔, 東山哲也, 植田美那子
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド、兵庫
  • Year and Date: 2015-12-01
• [Presentation] シロイヌナズナ受精卵のイメージングとケミカルスクリーニング：細胞極性と不等分裂を制御する仕組みとは？ (2015)
  • Author(s): 植田美那子，木全祐資，栗原大輔，山田朋美，南保正和, 大川（西脇）妙子, 桑田啓子，梅田正明, 東山哲也
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 朱鷺メッセ：新潟コンベンションセンター, 新潟
  • Year and Date: 2015-09-06
• [Presentation] シロイヌナズナの受精卵極性と初期胚パターンの制御機構 (2015)
  • Author(s): 植田美那子, 木全祐資, 栗原大輔, 東山哲也
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-18
• [Presentation] Molecular dynamics of plant axis formation (2015)
  • Author(s): Minako Ueda, Yusuke Kimata, Daisuke Kurihara, Tetsuya Higashiyama
  • Organizer: The 1st CSRS-ITbM Joint Workshop
  • Place of Presentation: 愛知
  • Year and Date: 2015-01-07
• [Presentation] Transcriptional network underlying the apical-basal axis formation in plant embryos (2014)
  • Author(s): Minako Ueda, Tetsuya Higashiyama
  • Organizer: The 38th Naito Conference
  • Place of Presentation: 北海道
  • Year and Date: 2014-10-08
• [Presentation] シロイヌナズナ受精卵における細胞極性化過程のライブイメージング (2014)
  • Author(s): 植田美那子，木全祐資，栗原大輔, 東山哲也
  • Organizer: エンドメンブレンミーティングプログラム
  • Place of Presentation: 愛知
  • Year and Date: 2014-09-30
• [Presentation] シロイヌナズナの受精卵極性とパターン形成の制御機構 (2014)
  • Author(s): 植田美那子，木全祐資，東山哲也
  • Organizer: 日本植物学会
  • Place of Presentation: 神奈川
  • Year and Date: 2014-09-12
• [Presentation] シロイヌナズナの胚発生制御因子の同定に向けた化合物スクリーニング (2014)
  • Author(s): 木全祐資, 佐藤綾人, 東山哲也, 植田美那子
  • Organizer: 日本植物形態学会
  • Place of Presentation: 神奈川
  • Year and Date: 2014-09-11
• [Presentation] Molecular dynamics of plant axis formation (2014)
  • Author(s): Minako Ueda, Yuusuke Kimata, Tetsuya Higashiyama
  • Organizer: International ERATO Higashiyama Live-Holonics Symposium 2014
  • Place of Presentation: 愛知
  • Year and Date: 2014-09-09
• [Presentation] Integration of paternal and maternal factors directly regulates transcription of Arabidopsis embryo patterning gene WOX8 (2014)
  • Author(s): Minako Ueda, Zhongjuan Zhang, Ernst Aichinger, Tetsuya Higashiyama, Masaaki Umeda, Edwin Groot and Thomas Laux
  • Organizer: 名古屋大学・フライブルグ大学共催ミニシンポジウム
  • Place of Presentation: ドイツ
  • Year and Date: 2014-06-17
• [Presentation] Molecular Regulation of Plant Axis Formation (2014)
  • Author(s): Minako Ueda, Yuusuke Kimata, Ayato Sato, Tetsuya Higashiyama
  • Organizer: The 2nd International Symposium on Transformative Bio-Molecules 2014
  • Place of Presentation: 愛知
  • Year and Date: 2014-05-12