Elucidation of biologically active laminarin-structure and development of its enzymatic evaluation method

• Project/Area Number: 26850129
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Aquatic life science
• Research Institution: Hokkaido University
• Principal Investigator: Kumagai Yuya 北海道大学, (連合)農学研究科(研究院), 研究員 (00589997)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: ラミナリナーゼ / オリゴ糖 / ラミナリン / 糖質結合モジュール / 糖質加水分解酵素 / GH64 / ラミナリオリゴ糖

Outline of Final Research Achievements

Laminarin has biologically active saccharides such as the immunostimulation and antitumor activiy. The laminarin structure differes from spicies, indicating that the relationship of the structures and the expression mechanism of bioactivities is still unclear. In this study, we employed glucanase with a limited hydrolysis activity toward laminarin and attempted for oligosaccharide production. The enzyme produced biologically active oligosaccharides and revealed the hydrolysis pattern of biologically active laminarin.

Research Products

• [Int'l Joint Research] Technical University of Denmark(Denmark)
• [Journal Article] Heat treatment of curdlan enhances the enzymatic production of biologically active β-(1,3)-glucan oligosaccharides (2016)
  • Author(s): Kumagai Y, Okuyama M, Kimura A
  • Journal Title: Carbohtdr. Polym. Volume: - Pages: 396-401
  • DOI: 10.1016/j.carbpol.2016.03.066
  • Peer Reviewed
• [Journal Article] The loop structure of Actinomycete glycoside hydrolase family 5 mannanases governs substrate recognition (2015)
  • Author(s): Kumagai Y, Yamashita K, Tagami T, Uraji M, Wan K, Okuyama M, Yao M, Kimura A, Hatanaka T
  • Journal Title: FEBS J. Volume: 282 Issue: 20 Pages: 4001-4014
  • DOI: 10.1111/febs.13401
  • NAID: 120005906892
  • Peer Reviewed / Open Access
• [Journal Article] Biochemical properties and substrate recognition mechanism of GH31 α-glucosidase from Bacillus sp. AHU 2001 with broad substrate specificity (2015)
  • Author(s): Wataru Saburi, Masayuki Okuyama, Yuya Kumagai, Atsuo Kimura, Haruhide Mori
  • Journal Title: Biochimie Volume: 108 Pages: 140-148
  • DOI: 10.1016/j.biochi.2014.11.010
  • Peer Reviewed
• [Journal Article] Different molecular complexity of linear-isomaltomegalosaccharides and β-cyclodextrin on enhancing solubility of azo dye ethyl red: Towards dye biodegradation (2014)
  • Author(s): Weeranuch Lang, Yuya Kumagai, Juri Sadahiro, Janjira Maneesan, Masayuki Okuyama, Haruhide Mori, Nobuo Sakairi, Atsuo Kimura
  • Journal Title: Bioresource Technology Volume: 169 Pages: 518-524
  • DOI: 10.1016/j.biortech.2014.07.025
  • Peer Reviewed
• [Presentation] Solubilizing ability of double-anchor type isomaltomegalosaccharide for drug delivery of ibuprofen (2016)
  • Author(s): Sota Uekusa, Weeranuch Lang, Yuya Kumagai, Masayuki Okuyama, Atsuo Kimura
  • Organizer: 日本農芸化学会(2015年度大会)
  • Place of Presentation: 北海道、札幌市・札幌コンベンションセンター
  • Year and Date: 2016-03-27
• [Presentation] Enzymatic synthesis of functional linear isomaltomegalosaccharide by Gluconobacter oxydans dextran dextrinase (2015)
  • Author(s): Yuya Kumagai, Weeranuch Lang, Juri Sadahiro, Masayuki Okuyama, Haruhide Mori, and Atsuo Kimura
  • Organizer: 11th Carbohydrate Bioengineering Meeting (CBM11)
  • Place of Presentation: Espoo, Finland
  • Year and Date: 2015-05-10
  • Int'l Joint Research
• [Presentation] 生理活性を持つβ‐１，３－グルカンオリゴ糖の酵素的調製法の検討 (2015)
  • Author(s): 熊谷祐也, 奥山正幸, 木村淳夫
  • Organizer: 日本農芸化学会2015年度大会
  • Place of Presentation: 岡山県、岡山市、岡山大学津島キャンパス
  • Year and Date: 2015-03-28
• [Presentation] 放線菌マンナナーゼの基質特異性に関するループ7の解析 (2014)
  • Author(s): 熊谷祐也, 裏地美杉, 奥山正幸, 木村淳夫, 畑中唯史
  • Organizer: 日本応用糖質科学会平成26年度大会
  • Place of Presentation: 新潟県、新潟市、朱鷺メッセ新潟コンベンションセンター
  • Year and Date: 2014-09-25 – 2014-09-26
• [Presentation] 放線菌マンナナーゼの分枝オリゴ糖に対する基質特異性に関する解析 (2014)
  • Author(s): 熊谷祐也, 裏地美杉, 奥山正幸, 木村淳夫, 畑中唯史
  • Organizer: 第６６回日本生物工学会
  • Place of Presentation: 北海道、札幌、札幌コンベンションセンター
  • Year and Date: 2014-09-10