The inhibitory mechanism of AVP release under hypoosmotic conditions

• Project/Area Number: 26860153
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General physiology
• Research Institution: Fukuoka University (2015-2017); National Institute for Physiological Sciences (2014)
• Principal Investigator: Sato-Numata Kaori 福岡大学, 医学部, 特別研究員(RPD) (60614196)
• Research Collaborator: OKADA Yasunobu 京都府立医科大学, 医学部, 特任教授 (10025661)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ＡＶＰニューロン / 低浸透圧 / タウリン / アストロサイト / イオンチャネル / 細胞容積 / バソプレシン / アニオンチャネル / 阻害剤 / バソプレシンニューロン

Outline of Final Research Achievements

We checked substances secreted under hypotonic conditions using primary cultured astrocytes collected from the rat SON region, and found that glutamic acid and aspartic acid are secreted in addition to taurine. Exposure of taurine or GABA to vasopressin neurons under hypotonic conditions suppressed spontaneous excitation despite membrane depolarization. The effect on membrane potential and spontaneous excitation by taurine exposure was abolished with inhibitors of GABAA receptors or glycine receptors but was not affected by inhibitors of GABAB receptors. From these results suggest ed that the effect on taurine to membrane depolarization and suppression of spontaneous excitation is occurred via glycine receptor and GABAA receptors.

Research Products

• [Journal Article] Cellular mechanism for herbal medicine Junchoto to facilitate intestinal Cl-/water secretion that involves cAMP-dependent activation of CFTR (2018)
  • Author(s): Tomohiro Numata, Kaori Sato-Numata, Yasunobu Okada, Ryuji Inoue
  • Journal Title: Journal of Natural Medicines Volume: 印刷中 Issue: 3 Pages: 694-705
  • DOI: 10.1007/s11418-018-1207-9
  • Peer Reviewed / Open Access
• [Journal Article] Distinct contributions of LRRC8A and its paralogs to the VSOR anion channel from those of the ASOR anion channel (2017)
  • Author(s): Kaori Sato-Numata, Tomohiro Numata, Ryuji Inoue, Ravshan Z. Sabirov and Yasunobu Okada.
  • Journal Title: Channels Volume: 11(2) Issue: 2 Pages: 167-172
  • DOI: 10.1080/19336950.2016.1230574
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Distinct pharmacological and molecular properties of the acid-sensitive outwardly rectifying (ASOR) anion channel from those of the volume-sensitive outwardly rectifying (VSOR) anion channel (2016)
  • Author(s): Kaori Sato-Numata, Tomohiro Numata, Ryuji Inoue, Yasunobu Okada
  • Journal Title: Pflugers Archiv - European Journal of Physiology Volume: 印刷中 Issue: 5 Pages: 795-803
  • DOI: 10.1007/s00424-015-1786-1
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Temperature sensitivity of acid-sensitive outwardly rectifying (ASOR) anion channels in cortical neurons is involved in hypothermic neuroprotection against acidotoxic necrosis. (2014)
  • Author(s): Sato-Numata K, Numata T, Okada Y
  • Journal Title: Channels (Austin) Volume: 8 Issue: 3 Pages: 278-283
  • DOI: 10.4161/chan.27748
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Properties and roles of flufenamate-sensitive ion channels stimulated by hyperosmolality in vasopressin neurons (2018)
  • Author(s): 佐藤(沼田)かお理, 沼田朋大，上田陽一, 井上隆司，岡田泰伸
  • Organizer: 第95回 日本生理学会大会
• [Presentation] LRRC8ファミリーは容積感受性外向整流性アニオンチャネル（VSOR）の活性に関与するが酸感受性外向整流性アニオンチャネル（ASOR）には関与しない (2017)
  • Author(s): 佐藤(沼田)かお理, 沼田朋大, 井上隆司, サビロフ ラブシャン，岡田泰伸
  • Organizer: 日本生理学会
  • Place of Presentation: アクトシティ浜松（静岡県浜松市）
  • Year and Date: 2017-03-28
• [Presentation] 酸感受性外向整流性アニオンチャネル（ASOR）と容積感受性外向整流性アニオンチャネル（VSOR）の薬理学的分別 (2016)
  • Author(s): 佐藤（沼田）かお理、沼田朋大、井上隆司、岡田泰伸
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 札幌コンベンションセンター （北海道札幌市）
  • Year and Date: 2016-03-22
• [Presentation] 酸感受性外向整流性Cl－チャネル（ASOR）と容積感受性外向整流性Cl－チャネル（VSOR）の薬理学的相違 (2015)
  • Author(s): 佐藤（沼田）かお理、沼田朋大、井上隆司、岡田泰伸
  • Organizer: 第68回日本薬理学会西南部会
  • Place of Presentation: 海峡メッセ下関（山口県下関市）
  • Year and Date: 2015-11-21
• [Presentation] ニューロン酸感受性外向整流性アニオンチャネル（ASOR）のアシドーシス性脳神経細胞障害に対する低温救済への関与 (2015)
  • Author(s): 佐藤（沼田）かお理、沼田朋大、井上隆司、岡田泰伸
  • Organizer: 第66回西日本生理学会
  • Place of Presentation: 久留米大学筑水会館（福岡県久留米市）
  • Year and Date: 2015-10-09
• [Presentation] Exploration of temperature sensitivity and new antagonists of acid-sensitive outwardly rectifying anion channel (ASOR) (2015)
  • Author(s): Kaori Sato-Numata, Tomohiro Numata, Yasunobu Okada
  • Organizer: 第92回 日本生理学会大会
  • Place of Presentation: 神戸国際会議場・展示場 （神戸ポートアイランド）(兵庫県神戸市)
  • Year and Date: 2015-03-21 – 2015-03-23
• [Remarks]
  • URL: http://www.med.fukuoka-u.ac.jp/physiol/study6.html