Ribosomal RNA gene mutation in Human Malignancies

• Project/Area Number: 26860233
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Human pathology
• Research Institution: Niigata University
• Principal Investigator: OHASHI Riuko 新潟大学, 医歯学系, 助教 (20447600)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: リボソーム遺伝子 / LXRα / 肺癌 / 遺伝子変異 / 遺伝子多型 / リボソームDNA / LXR / 核内受容体

Outline of Final Research Achievements

In this study, we identified new somatic mutations in proximity to ribosomal RNA gene transcription start site in two cases (5%) of 40 lung cancer patients by PCR-direct sequence method. To determine the relationship between clinicopathological significance and ribosomal RNA gene mutation, sequence analysis of more patients are ongoing.

Research Products

• [Int'l Joint Research] University of Zurich and University Hospital Zurich(スイス)
• [Journal Article] Frequent Germline and Somatic Single Nucleotide Variants in the Promoter Region of the Ribosomal RNA Gene in Japanese Lung Adenocarcinoma Patients (2020)
  • Author(s): Ohashi R, Umezu H, Sato A, Abテゥ T, Kondo S, Daigo K, Sato S, Hara N, Miyashita A, Ikeuchi T, Motoyama T, Kishi M, Nagaoka T, Horiuchi K, Shiga A, Okuda S, Sekiya T, Ohtsubo A, Ichikawa K, Kagamu H, Kikuchi T, Watanabe S, Tanuma JI, Schraml P, Hamakubo T, Tsuchida M, Ajioka Y.
  • Journal Title: Cells Volume: 9 Issue: 11 Pages: 2409-2409
  • DOI: 10.3390/cells9112409
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] LXRαの核小体への局在とリボソームDNA転写制御 (2015)
  • Author(s): 大橋 瑠子
  • Journal Title: 新潟医学会雑誌 Volume: 128 Pages: 447-461
  • NAID: 120006759125
  • Peer Reviewed
• [Presentation] 肺腺癌におけるリボソームRNA遺伝子プロモーター領域の遺伝子変異 Single Nucleotide Variants in the Promoter Region of the Ribosomal RNA Gene in Lung Adenocarcinoma (2021)
  • Author(s): 大橋 瑠子、梅津 哉、阿部 達也、近藤 修平、他
  • Organizer: 第110回日本病理学会総会
• [Presentation] 核内受容体LXRαの核小体への局在とリボソームDNA転写制御 (2015)
  • Author(s): 大橋 瑠子、梅津 哉、長谷川 剛, 八神 淑英、内藤 眞、近藤 英作
  • Organizer: 第104回日本病理学会総会
  • Place of Presentation: 名古屋国際会議場 (愛知県名古屋市)
  • Year and Date: 2015-04-30