In vivo patch-clamp analysis of central cytokine action on visceral functions

• Project/Area Number: 26860391
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pain science
• Research Institution: National Institute for Physiological Sciences
• Principal Investigator: AKIMOTO Nozomi 生理学研究所, 生体情報研究系, 特別協力研究員 (60723905)
• Co-Investigator(Renkei-kenkyūsha): FURUE Hidemasa 生理学研究所, 生体情報研究系, 准教授 (20304884)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: サイトカイン / 排尿 / 神経障害性疼痛 / 下部尿路 / ケモカイン / 脊髄

Outline of Final Research Achievements

Cytokines (TNF-alpha, IL-1 beta and CCL-1) are known to be involved in the development of neuropathic pain. However effects of the cytokines on spino-bulbospinal micturition reflex circuit remain unclear.
Application of IL-1 beta and CCL-1 to the spinal cord decreased inter-contraction interval. On the contrary, application of TNF-alpha increased it. Furthermore, we showed that efferent signal to bladder and urethra was transmitted from the same autonomic nucleus. However, afferent information from these organs was transmitted to the spinal cord by different afferent neurons. These data indicated that sensory afferent information such as pressure and chemicals from the lower urinary tract was separately transmitted to distinct neuronal populations in the spinal dorsal horn, and cytokines in the spinal cord control micturition reflex.

Research Products

• [Journal Article] Stimulation of dopamine D2-like receptors in the lumbosacral defecation centre causes propulsive co lorectal contractions in rats. (2016)
  • Author(s): Kiyotada Naitou, Hiroyuki Nakamori, Takahiko Shiina, Azusa Ikeda, Yuuta Nozue, Yuuki Sano, Takuya Yokoyama, Yoshio Yamamoto, Akihiro Yamada, Nozomi Akimoto, Hidemasa Furue, and Yasutake Shimizu
  • Journal Title: The Journal of Physiology Volume: 594 Issue: 15 Pages: 4339-4350
  • DOI: 10.1113/jp272073
  • Peer Reviewed / Open Access
• [Journal Article] Effects of 3,3',5-triiodothyronine on microglial functions (2015)
  • Author(s): Mori Y, Tomonaga D, Kalashnikova A, Furuya F, Akimoto N, Ifuku M, Okuno Y, Beppu K, Fujita K, Katafuchi T, Shimura H, Churilov LP, Noda M
  • Journal Title: Glia Volume: 63(5) Issue: 5 Pages: 906-920
  • DOI: 10.1002/glia.22792
  • Peer Reviewed / Open Access
• [Presentation] 下部尿路カプサイシン刺激に対するin vivo 髄後角痛覚応答 (2016)
  • Author(s): 秋元 望, 箱崎敦志, 河谷正仁, 井本敬二, 古江秀昌
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 札幌コンベンションセンター (北海道札幌市)
  • Year and Date: 2016-03-23
• [Presentation] 自律神経系を介した内の中枢性制御機構 (2016)
  • Author(s): 秋元 望
  • Organizer: 新潟脳研-生理研合同シンポジウム
  • Place of Presentation: 岡崎カンファレンスセンター (愛知県岡崎市）
  • Year and Date: 2016-03-01
• [Presentation] 下部尿路から脊髄に入力する痛覚伝達機構の in vivo 解析 (2015)
  • Author(s): 秋元望, 箱崎敦志, 河谷正仁, 井本敬二, 古江秀昌
  • Organizer: 第37回日本疼痛学会
  • Place of Presentation: 市民会館崇城大学ホール (熊本県熊本市)
  • Year and Date: 2015-07-04
• [Presentation] In vivo analysis of afferent pathways to the spinal cord from the bladder and urethra. (2015)
  • Author(s): Akimoto Ｎ, Hakozaki A, Imoto K, Furue H
  • Organizer: 第92回日本生理学会大会
  • Place of Presentation: 神戸交際会議場・展示場（兵庫県神戸市）
  • Year and Date: 2015-03-23
• [Presentation] Action of Chemokine (C-C motif) ligand 1 on synaptic transmission in the spinal superficial dorsal horn. (2014)
  • Author(s): Akimoto N
  • Organizer: 2014NIPS-KU/YU Symposium.
  • Place of Presentation: 岡崎カンファレンスセンター（愛知県岡崎市）
  • Year and Date: 2014-07-01
• [Presentation] 脊髄後角シナプス伝達に対するCCL-1の作用と疼痛発現機構 (2014)
  • Author(s): 秋元 望, 歌 大介, 本多健治, 高野行夫, 井本敬二, 野田百美, 古江秀昌
  • Organizer: 第36回日本疼痛学会
  • Place of Presentation: KKRホテル大阪（大阪府大阪市）
  • Year and Date: 2014-06-20