Analysis of skin microbiome using NGS technology and development of novel treatment

• Project/Area Number: 26860902
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Okayama University (2015-2016); Kinki University (2014)
• Principal Investigator: Shuta Tomida 岡山大学, 医歯薬学総合研究科, 准教授 (10372111)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 細菌叢 / NGS / 皮膚 / ニキビ / 比較ゲノム / 次世代シーケンサー / メタゲノム解析 / 比較ゲノム解析 / パンゲノム解析 / マイクロバイオーム / 次世代シークエンサー / アクネ菌 / プロバイオティクス / 共生細菌叢

Outline of Final Research Achievements

We have developed a series of protocol to analyze skin microbiome with highly reproducible manner using NGS, providing the basic idea of skin microbiome taken from Japanese people. In addition, based on the highly accurate draft genomes of the bacteriophages of Propionibacterium acnes, we succeeded in extraction of unique genome sequences that could affect the sensitivity to the phage infection. These results could shed light on the development of novel treatment against drug resistant P. acnes

Research Products

• [Journal Article] Comparative genome analyses of Mycobacterium avium reveal genomic features of its subspecies and strains that cause progression of pulmonary disease. (2017)
  • Author(s): Uchiya KI, Tomida S, Nakagawa T, Asahi S, Nikai T, Ogawa K.
  • Journal Title: Sci Rep. Volume: 7 Issue: 1 Pages: 39750-39750
  • DOI: 10.1038/srep39750
  • Peer Reviewed / Open Access
• [Journal Article] Draft Genome Sequence of Probiotic Lactobacillus acidophilus Strain L-55 Isolated from a Healthy Human Gut. (2016)
  • Author(s): Fujii Y, Toh H, Matsubara T, Tomida S, Nguyen CT, Mimura I, Nakamura S, Morita H.
  • Journal Title: Genome Announc. Volume: 4 Issue: 6
  • DOI: 10.1128/genomea.01357-16
  • Peer Reviewed / Open Access
• [Journal Article] Epidermolysis bullosa simplex with mottled pigmentation with noncicatricial alopecia: identification of a recurrent p.P25L mutation in KRT5 in four affected family members. (2016)
  • Author(s): Nagai H, Oiso N, Tomida S, Sakai K, Fujiwara S, Nakamachi Y, Kawano S, Kawada A, Nishio K, Nishigori C.
  • Journal Title: Br J Dermatol. Volume: 174 Issue: 3 Pages: 633-635
  • DOI: 10.1111/bjd.14083
  • Peer Reviewed
• [Journal Article] The diversity and host interactions of Propionibacterium acnes bacteriophages on human skin (2015)
  • Author(s): Jared Liu, Riceley Yan, Qiao Zhong, Sam Ngo, Nathanael J Bangayan, Lin Nguyen, Timothy Lui, Minghsun Liu, Marie C Erfe, Noah Craft, Shuta Tomida and Huiying Li
  • Journal Title: ISME J (advance online publication) Volume: 9 Issue: 9 Pages: 2078-2093
  • DOI: 10.1038/ismej.2015.47
  • Peer Reviewed
• [Presentation] 皮膚マイクロバイオーム解析 (2016)
  • Author(s): 冨田秀太
  • Organizer: 日本皮膚科学会総会
  • Place of Presentation: 京都
  • Year and Date: 2016-06-04
  • Invited