Relationship of ceraide in cellular response of heavy ion irradiation

Research Project

Project/Area Number	26860977
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Radiation science
Research Institution	Gunma University
Principal Investigator	Kawamura Hidemasa 群馬大学, 大学院医学系研究科, 講師 (80431716)
Project Period (FY)	2014-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000) Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	セラミド / バイスタンダー効果
Outline of Final Research Achievements	X-ray irradiation was applied to fibroblast cell line and chondrosarcoma cell line and co-cultured the unirradiated cells with irradiated cells to analyze cell response to radiation. In particular, in order to analyze the involvement of ceramide in cellular response of co-cultured non-irradiated cells, DNA damage and quantitative measurement of ceramide were carried out by image analysis using fluorescent immunostaining method. Radiation response of non-irradiated cells (bystander effect) was confirmed and fundamental studies were conducted to investigate the influence of ceramide.

Report

(4 results)