Search for lung cancer stem cells in pulmonary venous blood in primary lung cancer.

• Project/Area Number: 26861131
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Respiratory surgery
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: YONEDA Kazue 産業医科大学, 医学部, 助教 (80724806)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 原発性肺癌 / 肺静脈血 / 腫瘍細胞 / 循環腫瘍細胞

Outline of Final Research Achievements

Control of the recurrence of distant metastases after surgery is a major problem for improvement of prognosis after resection of primary lung cancer. There are not many cases where relapse or distant metastasis still occurs even if tumor can be completely resected. The involvement of circulating tumor cells in the blood is suggested for tumor hematogenous metastasis. We have quantitated the CTC of peripheral blood and pulmonary venous blood of lung cancer patients and found association with distant metastasis.
In this study, we aimed to find a new target for lung cancer treatment by trying to isolate lung cancer cells having proliferation and engraftment ability from the blood of the pulmonary vein leading from the lung to the general circulation, but this time in the lung cancer patients who were performed surgery, it is rarely seen that cancer cells in the pulmonary vein blood with self-proliferating ability outside the body.

Research Products

• [Journal Article] EpCAM-independent capture of circulating tumor cells with a 'universal CTC-chip' (2017)
  • Author(s): Chikaishi Y, Yoneda K, Ohnaga T, Tanaka F
  • Journal Title: ONCOLOGY REPORTS Volume: 37.1 Issue: 1 Pages: 77-82
  • DOI: 10.3892/or.2016.5235
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Circulating Tumor Cells as an Indicator of Postoperative Lung Cancer: A Case Report. (2017)
  • Author(s): Kuwata T, Yoneda K, Kobayashi K, Oyama R, Matumiya H, Shinohara S, Takenaka M, Oka S, Chikaishi Y, Inanishi N, Kuroda K, Tanaka F.
  • Journal Title: The American Journal of Case Reports Volume: 17 Pages: 663-665
  • DOI: 10.12659/ajcr.898934
  • Peer Reviewed
• [Journal Article] Circulating Tumor Cells: A Novel Detection System with “Universal” CTC-chip. (2017)
  • Author(s): Yoneda K, Tanaka F.
  • Journal Title: Proceedings of World Automation Congress Volume: - Pages: 631-665
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Capture of tumor cells in blood with “Universal CTC-chip”. (2017)
  • Author(s): Kazue Yoneda, Taiji Kuwata, Yasuhiro Chikaishi, Fumihiro Tanaka.
  • Organizer: 24th International Molecular Med TRI-CON 2017
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2017-02-19
  • Int'l Joint Research
• [Presentation] Universal CTC-chipによる血液中腫瘍細胞の捕捉 (2017)
  • Author(s): 米田和恵、桒田泰治、近石泰弘、由良冴希子、小林健一、小山倫太郎、松宮弘喜、金山雅俊、平良彰浩、名部裕介、篠原伸二、竹中 賢、岡 壮一、平井文子、田嶋裕子、今西直子、黒田耕志、大永 崇、田中文啓
  • Organizer: 第1回Liquid Biopsy研究会
  • Place of Presentation: 京王プラザホテル（東京都新宿区）
  • Year and Date: 2017-01-21
• [Presentation] Sensitive Detection of CTCs in Thoracic Malignant Tumors with "Universal" CTC-Chip. (2016)
  • Author(s): Kazue Yoneda, Taiji Kuwata, Yasuhiro Chikaishi, Kenichi Kobayashi, Rintaro Oyama R, Sakiko Yura, Hiroki Matsumiya, Akihiro Taira, Yusuke Nabe, Masaru Takenaka, Soichi Oka, Ayako Hirai, Yuko Tashima, Naoko Imanishi, Koji Kuroda, Takashi Ohnaga, Fumihiro Tanaka
  • Organizer: IASLC 17th World Conference on Lung Cancer
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2016-12-06
  • Int'l Joint Research
• [Presentation] “Universal CTC-chip”を用いた血液中腫瘍細胞の捕捉 第75回日本癌学会学術総会 (2016)
  • Author(s): 米田和恵、大永 崇、田中文啓
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-10-07
• [Presentation] “Universal CTC-chip”による血液中の中皮腫細胞捕捉 (2016)
  • Author(s): 米田和恵、桒田泰治、近石泰弘、由良冴希子、小林健一、小山倫太郎、松宮弘喜、平良彰浩、名部裕介、竹中 賢、岡 壮一、平井文子、田嶋裕子、今西直子、黒田耕志、田中文啓
  • Organizer: 第7回JMIG研究会
  • Place of Presentation: JPタワー名古屋ホール＆カンファランス（愛知県名古屋市）
  • Year and Date: 2016-09-03
• [Presentation] Circulating Tumor Cells: A Novel Detection System with “Universal” CTC-chip. (2016)
  • Author(s): Kazue Yoneda, Fumihiro Tanaka.
  • Organizer: World Automation Congress 2016 Japan Satellite Session
  • Place of Presentation: じばさんびる（兵庫県姫路市）
  • Year and Date: 2016-08-07
  • Int'l Joint Research
• [Presentation] “Universal CTC-chip”による血液中腫瘍細胞の捕捉 (2016)
  • Author(s): 米田和恵 近石泰弘、田中文啓
  • Organizer: 第1回CTC臨床応用研究会
  • Place of Presentation: 東大寺総合文化センター（奈良県奈良市）
  • Year and Date: 2016-07-17
• [Presentation] “Universal CTC-Chip”と抗ポドプラニン抗体NZ-1を用いた中皮腫細胞の捕捉 (2016)
  • Author(s): 米田和恵、加藤幸成、田中文啓
  • Organizer: 第20回日本がん分子標的治療学会
  • Place of Presentation: 別府国際コンベンションセンター（大分県別府市）
  • Year and Date: 2016-05-31
• [Presentation] Capture of EpCAM-negative mesothelioma cells with a “Universal CTC-chip”: Sensitivity tests and comparison with EpCAM-based standard method (2016)
  • Author(s): Kazue Yoneda, Yasuhiro Chikaishi, Takashi Ohnaga , Fumihiro Tanaka.
  • Organizer: AACR Annual Meeting 2016
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2016-04-19
  • Int'l Joint Research
• [Presentation] Improved efficacy in capturing EpCAM-negative tumor cells using a “Universal CTC-chip” (2015)
  • Author(s): Kazue Yoneda, Yasuhiro Chikaishi, Eri Kawashima, Takashi Ohnaga, Fumihiro Tanaka
  • Organizer: AACR Tumor metastasis 2015
  • Place of Presentation: Austin, USA
  • Year and Date: 2015-11-30
  • Int'l Joint Research
• [Presentation] 胸部悪性腫瘍におけるCell-based Liquid Biopsy (2015)
  • Author(s): 米田和恵
  • Organizer: 第75回日本呼吸器学会・日本結核病学会・日本サルコイドーシス/肉芽腫性疾患学会 九州支部秋季学術集会
  • Place of Presentation: ホテルグランデはがくれ（佐賀県佐賀市）
  • Year and Date: 2015-10-02
  • Invited
• [Presentation] “Universal CTC-Chip”によるEpCAM陰性循環腫瘍細胞の捕捉 (2015)
  • Author(s): 米田和恵、田中文啓
  • Organizer: 第19回日本がん分子標的治療学会学術集会
  • Place of Presentation: 松山全日空ホテル（愛媛県松山市）
  • Year and Date: 2015-06-10
• [Presentation] Capture of EpCAM-negative circulating tumor cells (CTCs) with a “Universal CTC-Chip” (2015)
  • Author(s): Kazue Yoneda, Yasuhiro Chikaishi, Eri Kawashima, Tomoko So, Hidetaka Uramoto, Takashi Ohnaga, Fumihiro Tanaka
  • Organizer: AACR Annual Meeting 2015
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 2015-04-18
  • Int'l Joint Research