New sarcoma therapy targeted to acidic microenvironment created by Vacuolar H+-ATPase (V-ATPase)
| Project/Area Number |
26861196
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | The University of Tokushima |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 酸性環境 / 肉腫 / V型ATPase / V-ATPase / pH / 細胞・組織特異性 / アイソフォーム / 脂肪腫 / 脂肪肉腫 |
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| Outline of Final Research Achievements |
To examine whether acidic microenvironment promote sarcoma activity, SW872 liposarcoma cells were cultured in pH7.4 or pH 6.4 medium. SW872 cells treated with acidic medium (pH 6.4) showed increased invasion and proliferation activity.
Next, We investigated expression of the vacuolar-type H-ATPase (V-ATPase) which has been shown to cause extracellular acidification by pumping protons. The isoform of a3, E2, F, G2, H were increased in liposarcoma (malignant tumor) compared by lipoma (benign tumor).
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Report
(3 results)
Research Products
(6 results)
