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New sarcoma therapy targeted to acidic microenvironment created by Vacuolar H+-ATPase (V-ATPase)

Research Project

Project/Area Number 26861196
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Orthopaedic surgery
Research InstitutionThe University of Tokushima

Principal Investigator

NISHISHO Toshihiko  徳島大学, 大学院医歯薬学研究部, 講師 (40444723)

Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords酸性環境 / 肉腫 / V型ATPase / V-ATPase / pH / 細胞・組織特異性 / アイソフォーム / 脂肪腫 / 脂肪肉腫
Outline of Final Research Achievements

To examine whether acidic microenvironment promote sarcoma activity, SW872 liposarcoma cells were cultured in pH7.4 or pH 6.4 medium. SW872 cells treated with acidic medium (pH 6.4) showed increased invasion and proliferation activity.
Next, We investigated expression of the vacuolar-type H-ATPase (V-ATPase) which has been shown to cause extracellular acidification by pumping protons. The isoform of a3, E2, F, G2, H were increased in liposarcoma (malignant tumor) compared by lipoma (benign tumor).

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (6 results)

All 2016 2015 Other

All Presentation (5 results) (of which Int'l Joint Research: 1 results) Remarks (1 results)

  • [Presentation] 脂肪肉腫細胞におけるプロトンポンプV型ATPaseの発現の意義2016

    • Author(s)
      宮城 亮、西庄俊彦、土岐俊一、西良浩一
    • Organizer
      第89回日本整形外科学会学術総会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-05-12
    • Related Report
      2015 Annual Research Report
  • [Presentation] 脂肪肉腫におけるプロトンポンプV型ATPaseの役割2016

    • Author(s)
      宮城 亮、西庄俊彦、土岐俊一、西良浩一
    • Organizer
      第126回中部日本整形外科災害外科学会
    • Place of Presentation
      アクトシティ浜松(静岡県浜松市)
    • Year and Date
      2016-04-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] The role of the vacuolar type H+-ATPase in the liposarcoma2016

    • Author(s)
      Ryo Miyagi, Toshihiko Nishisho, Shunichi Toki, Koichi Sairyo
    • Organizer
      2015 Annual meeting of the Orthopaedic Research Society
    • Place of Presentation
      Orland (USA)
    • Year and Date
      2016-03-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 脂肪肉腫細胞におけるプロトンポンプV型ATPaseの役割2015

    • Author(s)
      宮城 亮、西庄俊彦、土岐俊一、西良浩一
    • Organizer
      第30回 日本整形外科学会基礎学術集会
    • Place of Presentation
      富山国際会議場(富山県富山市)
    • Year and Date
      2015-10-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] 肉腫におけるプロトンポンプV型ATPaseの役割2015

    • Author(s)
      宮城 亮、西庄俊彦、土岐俊一、西良浩一
    • Organizer
      第125回中部日本整形外科災害外科学会
    • Place of Presentation
      ウインクあいち(愛知県名古屋市)
    • Year and Date
      2015-10-02
    • Related Report
      2015 Annual Research Report
  • [Remarks] 徳島大学運動機能外科学(整形外科) 研究

    • URL

      http://www.utokushima-orthop.com/research/

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

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