Contribution of TRPA1 to arousal and respiratory activation to hypoxia

• Project/Area Number: 26861234
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Anesthesiology
• Research Institution: Kagoshima University
• Principal Investigator: Kuroki Chiharu 鹿児島大学, 医学部・歯学部附属病院, 医員 (20725650)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: TRPA1 / 低酸素 / 鼻腔三叉神経 / 三叉神経 / 覚醒 / 呼吸

Outline of Final Research Achievements

We hypothesized that TRPA1 detect oxygen concentration in inspired gas and respiratory regulation, as early alarm system. We revealed that pathway trough TRPA1 contribute to arousal from sleep and respiratory activation, as a defensive response to hypoxia. Pathway trough TRPA1 suggested to locate on the trigeminal nerve in nasal cavity. This defense response is more important in mild hypoxia than severe hypoxia. We concluded TRPA1 is an early alarm system for hypoxia in airway.

Research Products

• [Journal Article] Intermittent but not sustained hypoxia activates orexin-containing neurons in mice. (2015)
  • Author(s): Keiji Yamaguchi, Takahiro Futatsuki, Jumpei Ushikai, Chiharu Kuroki, Toshiaki Minami, Yasuyuki Kakihana, Tomoyuki Kuwaki
  • Journal Title: Resp Physiol Neurobiol Volume: 206 Pages: 11-14
  • DOI: 10.1016/j.resp.2014.11.003
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 酸素および刺激ガスセンサーとしてのTRPA1チャンネルの役割 (2015)
  • Author(s): 桑木共之
  • Journal Title: 九州高気圧環境医学会誌 Volume: 15 Pages: 1-6
  • Peer Reviewed
• [Presentation] 捕食者回避における嗅神経とTRPA1含有三叉神経の役割 (2016)
  • Author(s): 赤星里佳、片山由璃子、山口蘭、Jordan Pauli、柏谷英樹、桑木共之
  • Organizer: 第11回環境生理プレコングレス
  • Place of Presentation: 北海道大学（北海道札幌市）
  • Year and Date: 2016-03-21
• [Presentation] 捕食者回避には嗅神経とTRPA1含有三叉神経が共に必要である (2015)
  • Author(s): 赤星里佳、片山由璃子、山口蘭、Jordan Pauli、柏谷英樹、桑木共之
  • Organizer: 第43回自律神経生理研究会
  • Place of Presentation: 日本光電（株）本社研修センター（東京都新宿区）
  • Year and Date: 2015-12-15
• [Presentation] Trigeminal TRPA1 contributes to irritant-induced respiratory slowing. (2015)
  • Author(s): Tomoyuki Kuwaki & Keiichi Inui
  • Organizer: NIPS international workshop 2015 "TRPs and SOCs ~Unconventional Ca2+ Physiology~".
  • Place of Presentation: Okazaki Conference Center（愛知県岡崎市）
  • Year and Date: 2015-06-04
• [Presentation] Mild hypoxia activates trigeminal neurons and wakes up sleeping mice though TRPA1 (2014)
  • Author(s): Chang Ping Chen, Chiharu Kuroki,Tomoyuki Kuwaki
  • Organizer: 第２４回日本病態生理学会
  • Place of Presentation: 北九州国際会議場（福岡県北九州市）
  • Year and Date: 2014-08-08 – 2014-08-10
• [Presentation] Mild hypoxia activates trigeminal neurons and wakes up sleeping mice though TRPA1 (2014)
  • Author(s): Chang Ping Chen, Chiharu Kuroki, Toru Yonemitsu, Shao Wu Ou, Tomoyuki Kuwaki
  • Organizer: 第４回鹿児島神経科学研究会
  • Place of Presentation: 鹿児島大学桜ヶ丘キャンパス（鹿児島県鹿児島市）
  • Year and Date: 2014-07-12
• [Presentation] 環境ガス早期警報装置としてのTRPA1 (2014)
  • Author(s): 桑木共之、米満亨、黒木千晴、高橋重成、森秦生
  • Organizer: TRP研究会2014
  • Place of Presentation: 生理学研究所（愛知県岡崎市）
  • Year and Date: 2014-06-05 – 2014-06-06