| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
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| Outline of Final Research Achievements |
We established an efficient biological design for the bacterial infection model; cylinder shaped chamber made of Poly dimethyl siloxane (PDMS), 100-200 um sized pores were added for immune cells infiltration into the device. Immunological investigation (e.g. Flowcytometry) for the collected cells and histomorphometric examination of biofilm formation which formed inside of the chamber were performed. The device showed an advantage especially for the analysis of neutrophil. Mixture of 4 common oral bacterial species ((P. intermedia (ATCC 25611), F. nucleatum (ATCC 25586), P. micros (ATCC 33270), S. intermedius (ATCC 27335)) showed thicker biofilm formation compared with the control species (Streptococcus mitis) at 24h time point after the infection. Surface preparation of the PDMS against for the exfoliation of biofilm formation seemed necessary for further structural analyses.
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