Development of periodontal tissue regenerative therapy by using clumps of MSCs/ECM complex

• Project/Area Number: 26861814
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Periodontology
• Research Institution: Hiroshima University
• Principal Investigator: Mikihito Kajiya 広島大学, 大学病院, 病院助教 (00633041)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 間葉系幹細胞 / 間葉系幹細胞集塊 / スキャフォールドフリー / C-MSC / 細胞治療 / MSC / ECM / 組織再生

Outline of Final Research Achievements

The transplantation of mesenchymal stem cells (MSCs) to damaged tissue has attracted attention in scientific and medical fields as an effective regenerative therapy. Nevertheless, additional studies are required to develop an MSC transplant method for bone regeneration because using an artificial scaffold restricts the number of transplanted cells and their function. Furthermore, regulating the degree of cell differentiation in vitro is desirable for a more effective regenerative therapy. To address these unresolved issues, by using self-produced extracellular matrix (ECM), we developed clumps of an MSC/ECM complex (C-MSCs). As a result, an applicant have demonstrated that C-MSC can be induced into osteogenic differentiation and its transplantation with no artificial scaffold facilitates effective bone regeneration in rat.
This result has been published in Cytotherapy.

Research Products

• [Journal Article] Clumps of a mesenchymal stromal cell/extracellular matrix complex can be a novel tissue engineering therapy for bone regeneration. (2015)
  • Author(s): Kittaka M, Kajiya M, Shiba H, Takewaki M, Takeshita K, Khung R, Fujita T, Iwata T, Nguyen TQ, Ouhara K, Takeda K, Fujita T, Kurihara H.
  • Journal Title: Cytotherapy. Volume: 2 Issue: 7 Pages: 48-61
  • DOI: 10.1016/j.jcyt.2015.01.007
  • Peer Reviewed / Open Access
• [Presentation] 三次元培養で実現する有効・安全な組織再生技術の新展開 (2015)
  • Author(s): 加治屋幹人
  • Organizer: 日本組織培養学会第88回大会
  • Place of Presentation: 広島
  • Year and Date: 2015-05-26
  • Invited
• [Presentation] 骨髄間葉系幹細胞集塊Clumps of mesenchymal stem cells/extracellular matrix complexを利用した新規組織再生療法開発 (2014)
  • Author(s): 加治屋幹人
  • Organizer: 第57回秋季日本歯周病学会学術大会
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2014-10-19