High efficiency differentiation of functional islets from human iPS cells and hiPS-endocrine progenitor cells in vitro.
| Project/Area Number |
26870158
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
General surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Watanabe Ami 東京大学, 分子細胞生物学研究所, 特任研究員 (40611421)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 膵臓 / iPS細胞 / 膵島 / iPS |
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| Outline of Final Research Achievements |
We previously showed that functional islets with a 3-dimensional structure consisting of multiple endocrine cells were formed in vitro from mouse fetal pancreatic bud cells. Based on this finding, we have developed a culture system to generate functional islet from human iPS cells. In this study, we isolate highly enriched population of pancreatic endocrine progenitor cells from endocrine progenitor cell culture stage cells by cell sorting. These cells can differentiate into the functional islet-like cell cluster. This result indicate that the iPS-islets can be generated from endocrine cells efficiently. Furthermore, we found that Ngn3 positive cells formed islet-like cell aggregate autonomously in this differentiation system. We are currently trying to scale up the culture system for production of a large quantity of islets.
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Report
(4 results)
Research Products
(6 results)
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[Presentation] Differentiation of functional islets from human iPS cells and hiPS-endocrine progenitor cells in vitro.2016
Author(s)
Ami Watanabe, Anna Tanaka, Hiroyuki Otsubo, Masahiro Ohta, Yzumi Yamashita-Sugahara, Kohnosuke Mitani, Mahito Nakanishi, Yasushi Okazaki, Atsushi Miyajima
Organizer
ISSCR
Place of Presentation
San francisco, California, USA
Year and Date
2016-06-22
Related Report
Int'l Joint Research
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