Functional regulation of cytoskeletal organization by a highly insoluble actin bundling protein Caprice

• Project/Area Number: 26870302
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry; Cell biology
• Research Institution: Kyoto University
• Principal Investigator: Kumeta Masahiro 京都大学, 生命科学研究科, 助教 (00582181)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 細胞骨格 / 生化学 / 細胞生物学 / アクチン / 分子生物学 / 天然変性領域

Outline of Final Research Achievements

The novel actin-bundling protein Caprice is characteristic in its 1) highly insoluble nature, 2) drastic effect on cellular morphology by over expression or knockdown, and 3) cell type-specific expression. We demonstrated that high concentration of Caprice protein in solution make it into a hydrogel, thus this protein is expected to confer specific property on the actin filament by undergoing sol-to-gel phase transition. Detailed analyses focusing on its transcriptional control and knock-down effect on cellular activities such as morphological regulation and adhesion properties reveled important roles of Caprice in organizing cytoskeletal dynamics.

Research Products

• [Journal Article] In vivo dynamics of the cortical actin network revealed by fast-scanning atomic force microscopy (2017)
  • Author(s): Yanshu Zhang, Aiko Yoshida, Nobuaki Sakai, Yoshitsugu Uekusa, Masahiro Kumeta, Shige H. Yoshimura
  • Journal Title: Microscopy Volume: 印刷中
  • NAID: 120006401728
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Dissecting in vivo steady-state dynamics of karyopherin-dependent nuclear transport. (2016)
  • Author(s): Lolodi O, Yamazaki H, Otsuka S, Kumeta M, Yoshimura SH.
  • Journal Title: Molecular Biology of the Cell Volume: 27 Issue: 1 Pages: 167-76
  • DOI: 10.1091/mbc.e15-08-0601
  • NAID: 120005997938
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Analyses of nuclear proteins and nucleic Acid structures using atomic force microscopy. (2015)
  • Author(s): Gilmore JL, Yoshida A, Takahashi H, Deguchi K, Kobori T, Louvet E, Kumeta M, Yoshimura SH, Takeyasu K
  • Journal Title: Methods in Molecular Biology Volume: 1262 Pages: 119-53
  • DOI: 10.1007/978-1-4939-2253-6_8
  • ISBN: 9781493922529, 9781493922536
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Probing in vivo dynamics of mitochondria and cortical actin networks using high-speed atomic force/fluorescence microscopy. (2015)
  • Author(s): Yoshida A, Sakai N, Uekusa Y, Deguchi K, Gilmore JL, Kumeta M, Ito S, Takeyasu K
  • Journal Title: Genes Cells Volume: 20(2) Issue: 2 Pages: 85-94
  • DOI: 10.1111/gtc.12204
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Caprice/MISP is a novel F-actin bundling protein critical for actin-based cytoskeletal reorganizations. (2014)
  • Author(s): Kumeta M., Gilmore J. L., Umeshima H., Ishikawa M., Kitajiri S., Horigome T., Kengaku M., and Takeyasu K
  • Journal Title: Genes Cells Volume: 19 Issue: 4 Pages: 338-349
  • DOI: 10.1111/gtc.12131
  • Peer Reviewed / Open Access
• [Journal Article] Structural mechanism of nuclear transport mediated by importin β and flexible amphiphilic proteins. (2014)
  • Author(s): Yoshimura SH, Kumeta M, Takeyasu K.
  • Journal Title: Structure Volume: 22(12) Issue: 12 Pages: 1699-710
  • DOI: 10.1016/j.str.2014.10.009
  • NAID: 120005515539
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Prolines in the α-helix confer structural flexibility on importin β necessary for nuclear transport (2016)
  • Author(s): Kumeta M, Konishi HA, Yoshimura SH.
  • Organizer: The 2016 American Society for Cell Biology Annual Meeting
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2016-12-03
  • Int'l Joint Research
• [Presentation] Nano-scale live cell imaging of cell cortex by fast-scanning atomic force microscope (2016)
  • Author(s): Yoshida A, Sakai N, Uekusa Y, Zhang Y, Kumeta M, Ito S, Yoshimura SH.
  • Organizer: The 39th Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: 横浜
  • Year and Date: 2016-11-30
• [Presentation] 高速原子間力顕微鏡による生体膜動態のライブセルイメージング (2016)
  • Author(s): 吉田藍子、酒井信明、植草良嗣、粂田昌宏、吉村成弘
  • Organizer: 第38回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 名古屋
  • Year and Date: 2016-11-17
• [Presentation] non-invasive imaging of membrane dynamics accompanied with endocytosis in living cells by atomic force microscopy (2016)
  • Author(s): Yoshida A, Sakai N, Uekusa Y, Kumeta M, Ito S, Yoshimura SH.
  • Organizer: 60th Annual Meeting of Biophysical Society
  • Place of Presentation: Los Angeles, USA
  • Year and Date: 2016-02-27
  • Int'l Joint Research
• [Presentation] 生細胞における皮質アクチンネットワークの動的分子イメージング (2016)
  • Author(s): 吉田藍子、酒井信明、植草良嗣、粂田昌宏、伊東修一、吉村成弘
  • Organizer: 第3回「分子ロボティクス」若手の会
  • Place of Presentation: 日本、京都
  • Year and Date: 2016-02-22
• [Presentation] Probing in vivo dynamics of memrane/cortical actin networks using high-speed atomic force/fluorescence microscopy (2015)
  • Author(s): Yoshida A, Sakai N, Uekusa Y, Kumeta M, Ito S, Yoshimura SH.
  • Organizer: The 38th Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: 日本、神戸
  • Year and Date: 2015-12-01
• [Presentation] Structural Analysis of Long Single Stranded RNA Molecules with Atomic Force Microscopy (2015)
  • Author(s): Gilmore JL, Yoshida A, Deguchi K, Asai S, Aizaki H, Kumeta M, Wakita T, Takeyasu K.
  • Organizer: Imaging 3rd International Multidisciplinary Microscopy and Microanalysis Congress and Exhibition
  • Place of Presentation: Ölüdeniz, Turkey
  • Year and Date: 2015-10-19
  • Int'l Joint Research
• [Presentation] Bidirectional cargo transport by importin ß (2015)
  • Author(s): Yamazaki H, Lolodi O, Yoshimura SH, Kumeta M.
  • Organizer: The 14th International Joint Mini-Symposium on Molecular and Cell Biology
  • Place of Presentation: 日本、京都
  • Year and Date: 2015-06-13
  • Int'l Joint Research
• [Presentation] Non-invasive imaging of F-actin dynamics in living cells by atomic force microsco (2015)
  • Author(s): Yoshida A, Deguchi K, Ito S, Kumeta M, Yoshimura SH.
  • Organizer: 59th Annual Meeting of Biophysical Society, USA
  • Place of Presentation: Baltimore, USA
  • Year and Date: 2015-02-07 – 2015-02-11
• [Presentation] Three-dimensional organization and dynamics of cortical actin networks in living cells revealed with high-speed atomic/ fluorescence microscopy (2014)
  • Author(s): Yoshida A, Deguchi K, Kumeta M, Yoshimura SH.
  • Organizer: The 37th Annual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Probing in vivo dynamics of mitochondria and cortical actin networks using high-speed atomic force/fluorescence microscopy (2014)
  • Author(s): Yoshida A, Ito S, Deguchi K, Gilmore J, Kumeta M, Takeyasu K.
  • Organizer: 13th NTU-KU-UT Joint Mini-Symposium
  • Place of Presentation: Tsukuba, Japan
  • Year and Date: 2014-09-26 – 2014-09-27