Construction of versatile flocculation system and its application for two-stage production system
Project/Area Number |
26870363
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Applied microbiology
Biofunction/Bioprocess
|
Research Institution | Kobe University |
Principal Investigator |
Tsuge Yota 神戸大学, 自然科学系先端融合研究環, 特命助教 (00647422)
|
Project Period (FY) |
2014-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | コリネ型細菌 / 有機酸 / アミノ酸 / フルフラール / 発酵 / コハク酸 / リジン / タンパク質工学 / 凝集 / アミノ酸生産 / 有機酸生産 |
Outline of Final Research Achievements |
We firstly investigated cell growth of amino acid producing Corynebacterium glutamicum under furfural stress. The growth was inhibited by furfural, but furfural was degraded into furfuryl alcohol and 2-furoic acid overtime. Through constructing deletion strains of alcohol dehydrogenase genes, we identified the gene responsible for the conversion of furfural into furfuryl alcohol. Purified protein of this gene converted furfural into furfuryl alcohol in vitro using NADPH as a co-factor. We moreover constructed a strain by metabolic engineering producing amino acid lysine under aerobic conditions and organic acid succinic acid under anaerobic conditions using oxygen as a metabolic switch.
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Report
(3 results)
Research Products
(12 results)