Analysis of mechanisms of wing development in hemimetabolous insect using conditional knock-out method.
| Project/Area Number |
26870415
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Insect science
Developmental biology
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| Research Institution | The University of Tokushima |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ゲノム編集 / ノックイン / ノックアウト / コンディショナルノックアウト / CRISPR/Cas9 system / コオロギ |
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| Outline of Final Research Achievements |
We attempted to establish the knock-in experiment mediated non-homorogous end joining repair pathway in the two spotted cricket. As a result, for the first time in the cricket, we succeeded to generate some knock-in lines inserted the expression cassette of marker gene. For conditional knock-out experiment, we constructed the knock-in vector containing component of piggybac system, and introduce into cricket genome. Furthermore, genome sequence analysis was performed by next generation sequencer PacBio RSII. We obtained about 5Gb data as long read sequence, and assemble to cricket genome sequence for Gap-closing analysis.
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Report
(3 results)
Research Products
(12 results)
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[Presentation] Genome editing in the two-spotted cricket, Gryllus bimaculatus, using CRISPR/Cas9 system.2015
Author(s)
Watanabe, T., Matsuoka, Y., Tomonari, S., Kurita, C., Noji, S., Mito, T.
Organizer
Insect Genetic Technologies WORKSHOP
Place of Presentation
Kansas, USA
Year and Date
2015-06-17
Related Report
Int'l Joint Research
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[Presentation] Genome sequencing and annotation of the cricket Gryllus bimaculatus, a hemimetabolous insect model.2015
Author(s)
Mito, T., Itoh, T., Mirimoto, H., Kijitani, R., Toyoda, A., Tomonari, S., Fuketa, M., Watanabe, T., Matsuoka, Y.
Organizer
9th Annual Arthropod Genomics SYMPOSIUM
Place of Presentation
Kansas, USA
Year and Date
2015-06-17
Related Report
Int'l Joint Research
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[Presentation] Functional analysis of a Hox gene, abdominal-A, using CRISPR/Cas9 system in the cricket Gryllus bimaculatus2015
Author(s)
Matsuoka, Y., Watanabe, T., Kurita, C., Tomonari, S., Noji, S., Mito, T.
Organizer
第４８回日本発生生物学会年会
Place of Presentation
つくば国際会議場（茨城県つくば市）
Year and Date
2015-06-02
Related Report
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[Presentation] Targeted genome editing in the cricket, Gryllus bimaculatus, using CRISPR/Cas9 system2014
Author(s)
Watanabe, T., Matsuoka, Y., Noji, S. and Mito, T.
Organizer
FASEB SRC on Genome Engineering - Cutting-Edge Research and Applications
Place of Presentation
Melia Nassau Beach, Nassau, Bahamas
Year and Date
2014-06-22 – 2014-06-27
Related Report
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