Study on the antitumor mechanism of Rb-dependent mitochondrial metabolic remodeling

• Project/Area Number: 26870443
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry; Tumor biology
• Research Institution: Mie University
• Principal Investigator: Takebayashi Shin-ichiro 三重大学, 医学(系)研究科(研究院), 講師 (50392022)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 細胞老化 / エネルギー代謝 / Retinoblastoma

Outline of Final Research Achievements

Cellular senescence is widely recognized as an important tumor suppressor function and accompanies metabolic remodeling. In this study, we demonstrate a novel role of Retinoblastoma (Rb) in oncogene-induced senescence. Our combined metabolic and gene expression analyses show that Rb mediates senescence-associated metabolic remodeling through transcriptional activation of a set of metabolic genes including glycolytic genes.

Research Products

• [Int'l Joint Research] University of Cambridge(英国)
• [Journal Article] Direct visualization of replication dynamics in early zebrafish embryos. (2016)
  • Author(s): Kuriya, K., Higashiyama, E., Avşar-Ban, E., Okochi, N., Hattori, K., Ogata, S., Takebayashi, S. I., Ogata, M., Tamaru, Y. and Okumura, K.
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 29 Pages: 1-4
  • Peer Reviewed
• [Journal Article] Retinoblastoma protein promotes oxidative phosphorylation through up-regulation of glycolytic genes in oncogene-induced senescent cells (2015)
  • Author(s): S. Takebayashi, H. Tanaka, S. Hino, Y. Nakatsu, T. Igata, A. Sakamoto, M. Narita, and M. Nakao.
  • Journal Title: Aging Cell Volume: 14 Issue: 4 Pages: 689-697
  • DOI: 10.1111/acel.12351
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Direct Visualization of DNA Replication Dynamics in Zebrafish Cells. (2015)
  • Author(s): Kuriya, K., Higashiyama, E., Avşar-Ban, E., Tamaru, Y., Ogata, S., Takebayashi, S., Ogata, M., and Okumura, K.
  • Journal Title: Zebrafish Volume: 12 Issue: 6 Pages: 432-439
  • DOI: 10.1089/zeb.2015.1151
  • Peer Reviewed
• [Presentation] 網膜芽細胞腫RBタンパク質は解糖系遺伝子を活性化することでがん遺伝子誘導性細胞老化における酸化的リン酸化を促進する (2016)
  • Author(s): 竹林慎一郎
  • Organizer: 第4回がんと代謝研究会
  • Place of Presentation: かごしま県民交流センター（鹿児島県鹿児島市）
  • Year and Date: 2016-07-07
  • Invited
• [Presentation] Retinoblastoma protein promotes oxidative phosphorylation through upregulation of glycolytic genes in oncogene-induced senescent cells. (2015)
  • Author(s): Shin-ichiro Takebayashi
  • Organizer: The 2nd International Symposium for Bioengineering Research and Education
  • Place of Presentation: UCSD campus, La Jolla, CA, USA
  • Year and Date: 2015-08-04
  • Int'l Joint Research
• [Presentation] BAFクロマチンリモデリング複合体による哺乳類染色体複製ドメイン構造の制御 (2014)
  • Author(s): 竹林慎一郎、緒方正人
  • Organizer: 第３７回日本分子生物学会年会（ワークショップ：ゲノムDNA複製制御のメカニズム：生物種を超えた統一像と多様性）
  • Place of Presentation: パシフィコ横浜、横浜
  • Year and Date: 2014-11-27
  • Invited
• [Presentation] BAFクロマチンリモデリング複合体による哺乳類染色体複製ドメイン構造の制御 (2014)
  • Author(s): 竹林慎一郎、緒方正人
  • Organizer: 第３７回日本分子生物学会年会（ポスター）
  • Place of Presentation: パシフィコ横浜、横浜
  • Year and Date: 2014-11-27
• [Presentation] ミトコンドリア活性に関わるエピジェネティック因子の探索 (2014)
  • Author(s): 田中宏、坂元顕久、竹林慎一郎、日野信次朗、中尾光善
  • Organizer: 第３７回日本分子生物学会年会（ポスター）
  • Place of Presentation: パシフィコ横浜、横浜
  • Year and Date: 2014-11-26
• [Presentation] BAFクロマチンリモデリング複合体による哺乳類染色体複製ドメイン構造の制御 (2014)
  • Author(s): 竹林慎一郎、緒方正人
  • Organizer: 第８回日本エピジェネティクス研究会年会（ポスター）
  • Place of Presentation: 伊藤国際学術研究センター、東京
  • Year and Date: 2014-05-26
• [Remarks] RBタンパク質は、老化細胞において解糖系遺伝子の活性化により酸化的リン酸化を促進する
  • URL: http://www.imeg.kumamoto-u.ac.jp/category/press/