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Studies on efficient utilization of pasture plant protein

Research Project

Project/Area Number 59360036
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 畜産化学
Research InstitutionNagoya University

Principal Investigator

TASAKI Iwao  School of Agriculture, Nagoya University, 農学部, 教授 (70023364)

Co-Investigator(Kenkyū-buntansha) UEDA Hiroshi  School of Agriculture, Kagawa University, 農学部, 助教授 (30116884)
HORIGOME Takao  School of Agriculture, Okayama University, 農学部, 教授 (00040824)
OKUMURA Jun-ichi  School of Agriculture, Nagoya University, 農学部, 助教授 (10023425)
OHSHIMA Mitsuaki  School of Agriculture, Nagoya University, 農学部, 助教授 (20035976)
KAYAMA Ryosei  School of Agriculture, Nagoya University, 農学部, 教授 (10031163)
Project Period (FY) 1984 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥10,100,000 (Direct Cost: ¥10,100,000)
Fiscal Year 1986: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1985: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1984: ¥5,600,000 (Direct Cost: ¥5,600,000)
KeywordsPasture plant protein / Leaf protein concentrate / 有効利用
Research Abstract

Legumes and forage grasses contain large amounts of protein, most of which may not be utilized efficiently by ruminants when given in the diet. An attempt was made, therefore, to extract excessive amounts of leaf protein and use the leaf protein concentrate more efficiently as a source of dietary protein for single-stomached animals. For this purpose, it is necessary to press juice from crushed grass followed by the precipitation of protein in the juice and drying the precipitated protein.
By comparing the precipitation methods, it was found that fermentation treatment caused a low recovery and inferior quality whereas high recovery was attained by alkali treatment, i. e. adjusting pH to 8 prior to heating. Heat-drying treatment of the precipitated protein led to the decomposition of amino acids, especially lysine. The mineral composition of the leaf protein concentrate was very different from that of an ordinary animal feed and was not well balanced for animal growth. By balancing the mineral composition, however, the utilization of the leaf protein concentrate was improved. Particularly notable was a high saponine content of the leaf protein concentrate extracted from alfalfa. When a large amount of saponine was included in the diet, chicks had severe growth retardation, which was completely counteracted by supplementing the diet with cholesterol at the same amount as saponine.
Besides these basic studies, a few practical experiments were also conducted. Without causing a detrimental effect on egg production, the leaf protein concentrate could be substituted for soybean meal in the diet for laying hens up to 5% on a protein basis, and even the supplementation was useful for gaining more yellowish color in the yolk. The leaf protein concentrate could be used as a protein source for the pigling diet at various amounts depending on the age.

Report

(1 results)
  • 1986 Final Research Report Summary
  • Research Products

    (52 results)

All Other

All Publications (52 results)

  • [Publications] Ohshima,M.;Y.Moriyama: Jpn.J.Zootech.Sci.56. 144-151 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ohshima.M.: Jpn.J.Zootech.Sci.56. 267-273 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kayama,R.;H.Tano;T.Kubota;T.Nagatomo;I.Tasaki: Proc.【XV】 Int.Grassl.Congr.849-850 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Horigome,T.;Y.S.Cho;S.Uchida;E.Sakaguchi: Proc.【XV】 Int.Grasst.Congr.857-859 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ohshima,M.;H.Ueda: Proc.【XV】 Int.Grassl.Congr.904-906 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ohshima,M.: Proc.【XV】Int.Grassl.Congr.907-909 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Terapuntuwat,S.;I.Tasaki: Proc.【XV】 Int.Grassl.Congr.912-913 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Cho,Y.S.;J.K.Kim;T.Horigome;S.Uchida: Proc.【XV】 Int.Grassl.Congr.921-922 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ohshima,M.;H.Ueda: Jpn.J.Zootech.Sci.57. 410-415 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ohshima,M.;H.Ueda: Jpn.J.Zootech.Sci.57. 416-421 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Terapuntuwat,S.;I.Tasaki: Jpn.J.Zootech.Sci.57. 430-437 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Terapuntuwat,S.;I.Tasaki: Jpn.J.Zootech.Sci.57. 524-533 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ohshima,M.;H.Ueda: Jpn.J.Zootech.Sci.57. 534-539 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Horigome,T.;Y.S.Cho;S.Uchida: Jpn.J.Zootech.Sci.57. 654-662 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ueda,H.;M.Ohshima;M.Kamada: Japan.Poult.Sci.23. 195-202 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ohshima,M: J.Japan.Grassl.Sci.32. 72-75 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ohshima,M.;V.A.Savangikar;C.V.Savangikar: J.Japan.Grassl.Sci.32. 205-210 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Terapuntuwat,S.;I.Tasaki: Nutr.Rep.Int.33. 747-752 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Terapuntuwat,S.;I.Tasaki: Jpn.J.Zootech.Sci.58. 80-86 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ohshima,M.;H.Nakamura;H.Tano;T.Okajima;R.Kayama: Proc.【IV】 AAAP Anim.Sci.Congr.(1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Horigome,T.: Proc.【IV】 AAAP Anim.Sci.Congr.(1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Terapuntuwat,S.;I.Tasaki: Proc.【IV】 AAAP Anim.Sci.Congr.(1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ueda,H.;M.Ohshima: Tech.Bull.Fac.Agr.Kagawa Univ.(1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ueda,H.;M.Ohshima;I.Akitomo: Jpn.J.Zootech.Sci.(1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ueda,H.;M.Ohshima: Jpn.J.Zootech.Sci.(1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Savangikar,C.V.;M.Ohshima: J.Agric.Food Chem.(1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ohshima, M. and Y. Moriyama: "Effect of ethanol treatment of heat-coagulated LPC before oven-drying on the nutritive value in rats." Jpn. J. Zootech. Sci.56. 144-151 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ohshima, M.: "The second limiting amino acid of ladino clover LPC in rats." Jpn. J. Zootech. Sci.56. 267-273 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kayama, R., H. Tano, T. Kubota, T. Nagatomo and I. Tasaki: "Feeding values of alfalfa and its dried press cake." Proc. <XV> Int. Grassl. Congr.849-850 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Horigome, T., Y. S. Cho, S. Uchida and E. Sakaguchi: "Effects of the leaf protein on serum cholesterol levels in rats." Proc. <XV> Int. Grassl. Congr.857-859 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ohshima, M. and H. Ueda: "Effect of anaerobic fermentation of plant juice on the yield and the nutritive value of leaf protein concentrate." Proc. <XV> Int. Grassl. Congr.904-906 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ohshima, M.: "Effect of drying method on the nutritive value of leaf protein concentrates coagulated by acidification, heat or anaerobic fermentation." Proc. <XV> Int. Grassl. Congr.907-909 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Terapuntuwat, S. and I. Tasaki: "Influence of the age of chicken on the metabolizable energy value of alfalfa leaf protein concentrate." Proc. <XV> Int. Grassl. Congr.912-913 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Cho, Y. S., J. K. Kim, T. Horigome and S. Uchida: "Effect of remaining brown juice in wet protein curd on the quality of leaf protein after drying." Proc. <XV> Int. Grassl. Congr.921-922 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ohshima, M. and H. Ueda: "Effect of fermentative coagulation on the yeild and the amino acid compositions of leaf protein concentrates." Jpn. J. Zootech. Sci.57. 410-415 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ohshima, M. and H. Ueda: "Nutritional evaluation of leaf protein concentrates coagulated by anaerobic fermentation of plant juice in rats." Jpn. J. Zootech. Sci.57. 416-421 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Terapuntuwat, S. and I. Tasaki: "Effects of alcohol treatment and supplementation of methionine or corn oil on nutritive value of alfalfa leaf protein concentrate for young chicks." Jpn. J. Zootech. Sci.57. 430-437 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Terapuntuwat, S. and I. Tasaki: "Effect of dietary saponin on the performance and plasma cholesterol level of chicks and the alleviation of saponin toxicity by cholesterol supplementation." Jpn. J. Zootech. Sci.57. 524-533 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ohshima, M. and H. Ueda: "Yield and nutritive value of LPC separated from Italian ryegrass juice stored for one or two days." Jpn. J. Zootech. Sci.57. 534-539 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Horigome, T., Y. S. Cho and S. Uchida: "Detrimental effect of remaining deproteinized juice in moist protein curd on the nutritive quality of leaf protein after hot-air drying." Jpn. J. Zootech. Sci.57. 654-662 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ueda, H., M. Ohshima and M. Kamada: "The effects of processing methods and cholesterol addition on the nutritive value of leaf protein concentrates in chicks." Japan. Poult. Sci.23. 195-202 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Horigome, T.: "Effects of alfalfa fractionated leaf proteins on serum cholesterol levels in the rat." Proc. <IV> AAAP Anim. Sci. Congr.(1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Terapuntuwat, S. and I. Tasaki: "Effect of alfalfa LPC saponin on the performance and plasma cholesterol level of chicks and alleviation of saponin toxicity by cholesterol supplementation." Proc. <IV> AAAP Anim. Sci. Congr.(1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ueda, H. and M. Ohshima: "Digestibility of leaf protein concentrates by in vitro method using pig intestinal fluid." Tech. Bull. Fac. Agr. Kagawa Univ.(1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ueda, H., M. Ohshima and I. Akitomo: "Nutritive value and hypocholesterolemic effect of alfalfa leaf protein concentrates prepared from two different varieties in chicks." Jpn. J. Zootech. Sci.(1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ueda, H. and M. Ohshima: "Effects of alfalfa saponin on chick performance and plasma cholesterol level." Jpn. J. Zootech. Sci.(1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ohshima, M.: "Comparison between lucerne and Italian ryegrass as the material crops fro LPC production." J. Japan. Grassl. Sci.32. 72-75 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ohshima, M., V. A. Savangikar and C. V. Savangikar: "Nutritive value of leaf protein products from lucerne prepared at pilot plant scale:Leaf protein concentrate, enriched wheat flour and calf milk replacer." J. Japan. Grassl. Sci.32. 205-210 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Terapuntuwat, S. and I. Tasaki: "Effect of mineral composition and soybean protein/alfalfa leaf protein concentrate of diets on chick growth" Nutr. Rep. Int.33. 747-752 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Terapuntuwat, S. and I. Tasaki: "Influence of cholesterol supplementation on protein and amino acid digestibilities of alfalfa leaf protein concentrate in cockerels." Jpn. J. Zootech. Sci.58. 80-86 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ohshima, M., H. Nakamura, H. Tano, T. Okajima and R. Kayama: "Effect of coagulation methods on the yeild and the quality of leaf protein concentrate." Proc. <IV> AAAP Anim. Sci. Congr.(1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Savangikar, C. V. and M. Ohshima: "Application of in vitro methods to assess the nutritive value of leaf protein concentrates." J. Agric. Food Chem.(1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

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Published: 1987-03-31   Modified: 2016-04-21  

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