Structure and Function of Self-replicate RNAs
| Project/Area Number |
59380025
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
分子遺伝学・分子生理学
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| Research Institution | University of Tokyo |
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Principal Investigator |
OKADA Yoshimi Faculty of Science, University of Tokyo, Professor, 理学部, 教授 (30011703)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAJIMA Katsuhisa Institute of Medical Science, University of Tokyo, Associate Professor, 医科学研究所, 助教授 (40012778)
SHIBUTA Hiroshi Institute of Medical Science, University of Tokyo, Professor, 医科学研究所, 教授 (70012721)
NOMOTO Akio Faculty of Medicine, University of Tokyo, Associate Professor, 医学部, 助教授 (70112670)
ISHIHAMA Akira National Institute of Genetics, Professor, 教授 (80019869)
SHIKATA Eishiro Faculty of Agriculture, Hokkaido University, Professor, 理学部, 教授 (00001389)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥24,600,000 (Direct Cost: ¥24,600,000)
Fiscal Year 1986: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1985: ¥9,300,000 (Direct Cost: ¥9,300,000)
Fiscal Year 1984: ¥9,300,000 (Direct Cost: ¥9,300,000)
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| Keywords | RNA / Viral RNA / Viroid / Replication of RNA / Genome structure / cDNAクローン |
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| Research Abstract |
An in vitro transcription system to produce infectious TMV RNA from a cloned cDNA has been established. Using this system, several mutations were introduced at or near the termination codon of the 130K protein. The 130K and 180K proteins are confirmed to be involved in viral RNA replication. The pathogenisities and nucleotide sequences of cucumber pale fruit viroid, grapevine viroid and hop stunt viroid was investigated and demonstrated that the pathogenisities of these three viroids are quite simialr, though some differences are recognized in their nucleotide sequences. Nucleotide sequence of apple scar skin viroid has been determined.
Multiple functions of the RNA-dependent RNA polymerase in influenza virus have been identified including the endonucleolvtic cleavage of capped RNA, the primer dependent RNA synthesis, the polyadenylation of transcripts and an apparent proofreading function. For studying the antigenic determinant sites or functional domain of sialic acid binding activity of influenza virus HA, site directed mutagenesis was applied. The genome organization of Sendai virus was elucidated completely and it was found that the nonstructural C protein of the virus plays an important role in induction of interferon. In vitro modified polioviruses were constructed by using the infectious cDNA clone. Biological tests including plaque size marker test and monkey neurovirulence test were performed on these mutant viruses. The results suggested that interactions between different genome loci of the RNA were importantly involved in the replication and gene expression.
The complete nucleotide sequence of the RNA coliphage GA and SP were determined. The amino acid residues in the coat protein causing the changes of the serological properties from GA to MS2 were identified.
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Report
(1 results)
Research Products
(13 results)
