Pathophysiological and therapeutic significance of regulation of hemopoietic cell differentiation and proliferation in hematological disorders
| Project/Area Number |
59440053
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Hematology
|
|---|
| Research Institution | University of Tokyo |
|---|
Principal Investigator |
TAKAKU Fumimaro University of Tokyo, 医学部, 教授 (40048955)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HIRAI Hisamaru University of Tokyo, 医学部, 助手 (90181130)
OKABE Tetsuro University of Tokyo, 医学部, 助手 (80169135)
KASUGA Masato University of Tokyo, 医学部, 助手 (50161047)
URABE Akio University of Tokyo, 医学部, 助手 (60142246)
OHSAWA Nakaaki University of Tokyo, 医学部, 助教授 (90010090)
|
|---|
| Project Period (FY) |
1984 – 1986
|
| Project Status |
Completed (Fiscal Year 1986)
|
| Budget Amount *help |
¥10,500,000 (Direct Cost: ¥10,500,000)
Fiscal Year 1986: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1985: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1984: ¥4,500,000 (Direct Cost: ¥4,500,000)
|
|---|
| Keywords | Leukemia / oncogene / growth factors / 分化増殖 / 分化 |
|---|
| Research Abstract |
We reported activation of the N-ras oncogene in leukemic cells of acute lymphocytic leukemia and chronic myelocytic leukemia patients. This activation was demonstrated by in vitro transfection assay using NIH 3T3 cells. Cloning and nucleotide analysis of the activated N-ras oncogene showed that the 34th nucleotide was changed to thymine from guanine by a point mutation. We also studied the transforming gene in bone marrow cells of patients with various hematopoietic malignancies, including myelodysplastic syndrome, by transplanting the transfected NIH 3T3 cells into nude mice. It was noted that in cases of primary acquired sideroblastic anemia, primary aquired refractory anemia, refractory anemia with excess of blasts and chronic myelomonocytic leukemia, the presence of the transforming gene was demonstrated in their bone marrow cells. These cases are considered to be in a state of leukemia even though they are diagnosed as preleukemia by the hematological findings.
|
Report
(2 results)
Research Products
(18 results)
