| Project/Area Number |
59840013
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
天然物有機化学
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
HIRATA Toshifumi Hiroshima University, 理学部, 助教授 (80033926)
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| Co-Investigator(Kenkyū-buntansha) |
AOKI Tadashi Suzugamine Women's college, 教授 (40112164)
HAMADA Hiroki Okayama University of Science, 理学部, 助手 (10164914)
MUNESADA Kiyotaka Hiroshima University, 理学部, 助手 (30166235)
TANIGUCHI Kenji Hiroshima University, 理学部, 講師 (10163627)
SUGA Takayuki Hiroshima University, 理学部, 教授 (00033811)
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| Project Period (FY) |
1984 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥9,100,000 (Direct Cost: ¥9,100,000)
Fiscal Year 1986: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1985: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1984: ¥6,300,000 (Direct Cost: ¥6,300,000)
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| Keywords | Plant cultured cells / Biotransformation / Shoot tip culture / Oxidoreduction / Stereospecificity / Enantioselectivity / Simulation of time-course / 茎頂培養 / タバコ培養細胞 |
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| Research Abstract |
The biochemical potentiality of plant cultured cells to produce useful substances is of considerable interest in connection with the use of the cultured cells as a bioreactor. We have investigated the bioconversion of terpenoids with the cultured cells of Nicotiana tabacum for generalizing the bioconversion pattern and the in vitro shoot tip cultures of Zingiber officinale and Mentha spicata for producing the volatile constituents by the cultured cells.
(1) The cultured cells were found to have the ability to transform the substrates as follows: the regio- and stereo-selective hydroxylation at the <alpha> -position of carbonyl group, the stereospecific hydrogenation of the C-C double bond adjacent to carbonyl group, and the enantioselective conversion of the hydroxyl, carbonyl, and acetoxyl groups and the C-C double bond.
(2) The oxidoreduction between cyclic alcohols and their corresponding ketones in the cultured cells of N. tabacum was governed by an <NAD^+> - dependent alcohol dehydrogenase and the balance in the equilibrium of the oxidoreduction was correlated with the <^(13)C> NMR chemical shift of the carbonyl carbon.
(3) The time-courses in the oxidoreduction in the cultured cells can be predicted on the basis of the permeability of the substrates into the cells and the <^(13)C> NMR chemical shifts of the carbonyl carbon.
(4) The accumulation of mono- and sesquiterpenoids in the culture plantlets of z. officinale and M. spicata can be controlled by the combination of the ouxin and the basal culture medium.
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