| Project/Area Number |
60430025
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
高分子合成
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| Research Institution | Hokkaido University |
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Principal Investigator |
TOKURA Seiichi Dept. of Polymer Sci., Faculty of Science, Hokkaido University, 理学部, 教授 (40000806)
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| Co-Investigator(Kenkyū-buntansha) |
NISHI Norio Dept. of Polymer Science, Faculty of Science, Hokkaido University, 理学部, 助手 (70001857)
金子 元三 北海道大学, 理学部, 教授 (10000720)
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| Project Period (FY) |
1985 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥21,000,000 (Direct Cost: ¥21,000,000)
Fiscal Year 1988: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1987: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1986: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1985: ¥13,500,000 (Direct Cost: ¥13,500,000)
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| Keywords | Deacetylated chitins / Carboxymethyl-chitins / Sulfated chitin derivatives / Immunoadjuvant activity / Hapten specific antibody / Controlled release / ハプテン特異抗体 / カルシウムイオン吸着 / アミノ酸特異吸着 / ゲル化 / 医薬トラッピング / プロドラッグ / カルボキシルメチルキチン / 特異抗体 / メタンフェタミン / 抗血栓性 / 酵素標識法 / キチン誘導体 / 生物活性 / 酵素標識抗体法 / ヒロポン / リンパ球親和性 / 選択吸着 / マクロファージ活性化 / 抗腫瘍活性 / 生物活性と置換位置 / マイトージェン活性 |
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| Research Abstract |
Various chitin derivatives were prepared to investigate the artificial control of antigenicity, biodegradabillity and blood compatibility for a biomedical applications. A relatively long-acting immunoadjuvant activity was achieced by 70% deacetylated chitin(DAC-70) among variously deacetylated chitins, and a short-acting immunoadjuvant was found in 80% carboxymethylated chitin(substituted at C-6 position of the GLcNAc residues) and also among variously substituted carboxymethyl(CM)-chitins. The immunoadjuvant activity of DACs seems to disappear upon carboxymethylation or O-N-sulfations. The immunoadjuvant property of CM-chitins was assumed to be fairly different from those of DACs and also disappeared by deacetylation, further carboxymethylation at the C-3 position, or by O-sulfation. CM-chitin was demonstrated to act as a hapten carrier which induced hapten specific antibody production in the presence of Freund's complete adjuvant, and as a controlled-release drug carrier in the absence of Freund's complete sdjuvant.
A 70% deacetylated-60%-CM-chitin was converted to a blood compatible, nonbiodegradable material when it was sulfated on the hydroxyl groups at C-6 and on some of the C-3 position. A preliminary test found no toxicity of these CM-chitin heparinoids, when they were injected into rat veins.
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