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Studies on the Syntheses of Bioactive Chitin Derivatives.

Research Project

Project/Area Number 60430025
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 高分子合成
Research InstitutionHokkaido University

Principal Investigator

TOKURA Seiichi  Dept. of Polymer Sci., Faculty of Science, Hokkaido University, 理学部, 教授 (40000806)

Co-Investigator(Kenkyū-buntansha) NISHI Norio  Dept. of Polymer Science, Faculty of Science, Hokkaido University, 理学部, 助手 (70001857)
金子 元三  北海道大学, 理学部, 教授 (10000720)
Project Period (FY) 1985 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥21,000,000 (Direct Cost: ¥21,000,000)
Fiscal Year 1988: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1987: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1986: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1985: ¥13,500,000 (Direct Cost: ¥13,500,000)
KeywordsDeacetylated chitins / Carboxymethyl-chitins / Sulfated chitin derivatives / Immunoadjuvant activity / Hapten specific antibody / Controlled release / ハプテン特異抗体 / カルシウムイオン吸着 / アミノ酸特異吸着 / ゲル化 / 医薬トラッピング / プロドラッグ / カルボキシルメチルキチン / 特異抗体 / メタンフェタミン / 抗血栓性 / 酵素標識法 / キチン誘導体 / 生物活性 / 酵素標識抗体法 / ヒロポン / リンパ球親和性 / 選択吸着 / マクロファージ活性化 / 抗腫瘍活性 / 生物活性と置換位置 / マイトージェン活性
Research Abstract

Various chitin derivatives were prepared to investigate the artificial control of antigenicity, biodegradabillity and blood compatibility for a biomedical applications. A relatively long-acting immunoadjuvant activity was achieced by 70% deacetylated chitin(DAC-70) among variously deacetylated chitins, and a short-acting immunoadjuvant was found in 80% carboxymethylated chitin(substituted at C-6 position of the GLcNAc residues) and also among variously substituted carboxymethyl(CM)-chitins. The immunoadjuvant activity of DACs seems to disappear upon carboxymethylation or O-N-sulfations. The immunoadjuvant property of CM-chitins was assumed to be fairly different from those of DACs and also disappeared by deacetylation, further carboxymethylation at the C-3 position, or by O-sulfation. CM-chitin was demonstrated to act as a hapten carrier which induced hapten specific antibody production in the presence of Freund's complete adjuvant, and as a controlled-release drug carrier in the absence of Freund's complete sdjuvant.
A 70% deacetylated-60%-CM-chitin was converted to a blood compatible, nonbiodegradable material when it was sulfated on the hydroxyl groups at C-6 and on some of the C-3 position. A preliminary test found no toxicity of these CM-chitin heparinoids, when they were injected into rat veins.

Report

(5 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • 1985 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] I.Azuma;J.Iida;K.Nishimura;C.Ishihara;S.Tokura;Y.Yamamura: Advances in the Biosciences. 68. 29-37 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Y.Uraki;S.Tokura: J.Macromol.Sci.-Chem.A25. 1427-1441 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 矢吹稔: "最後のバイオマス キチン・キトサン" 技報堂, 268 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] I. Azuma; J. Iida; K. Nishimura; C. Ishihara; S. Tokura; Y. Yamamura.: "Prevention of Sendai Virus Infection with Synthetic MDP and Chitin Derivatives in Mice." Advances in the Bioscience.68. 29-37 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Y. Uraki; S. Tokura: "Calcium-mediated Adsorption of Neutral Amino Acids to Carboxymethyl-chitin." J. Macromol. Sci. -Chem.A25. 1427-1441 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] M. Yabuki: Last Biomass Resource. Chitin. Chitosan. Giho Do, 268 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] I.Azuma;J.Iida;K.Nishimura;C.Ishihara;S.Tokura;Y.Yamamura: Advances in the Biosciences. 68. 29-37 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] Y.Uraki;S.Tokura: J.Macromol.Sci.-Chem.A25. 1427-1441 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] 矢吹稔: "最後のバイオマス キチン・キトサン" 技報堂, 268 (1988)

    • Related Report
      1988 Annual Research Report
  • [Publications] S. Tokura; S. Nishimura; N. Nishi; K. Nakamura; O. Hasegawa; H. Seo: S en-i Gakkaishi. 43. 288-293 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] N. Nishi; Y. Maekita; S. Nishimura; S. Tokura: International Journal of Biological Macromolecule. 9. 109-114 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] S. Tokura; O. Hasegawa; S. Nishimura; N. Nishi; T. Takatori: Analytical Biochemistry. 161. 117-122 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] S. Nishimura; S. Tokura: International Journal of Biological Macromolecule. 9. 225-232 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] S. Nishimura; N. Nishi; S. Tokura: International Journal of Biological Macromolecule. 9. 305-307 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] R. Suzuki; N. Nishi; S. Tokura; F. Morita: Journal of Biological Chemistry. 262. 11410 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 品田利彦(編): "キチン・キトサンの開発と応用" 工業技術会, 390 (1987)

    • Related Report
      1987 Annual Research Report
  • [Publications] 矢吹稔: "最後のバイオマス キチン・キトサン" 技報堂, 268 (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] S.Nishimura;N.Nishi;S.Tokura: Carbohydrate Research. 146. 251-258 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] K.Nishimura;S.Nishimura;H.Seo;N.Nishi;S.Tokura;I.Azuma: Journal of Biomedical Materials Research. 20. 1359-1372 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] S.Nishimura;N.Nishi;S.Tokura: Carbohyrate Research. 156. 286-292 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 戸倉清一: フードケミカル. 11. 29-35 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 戸倉清一,西村紳一郎: 蛋白質・核酸・酵素. 31-15. 1621-1631 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] International J.Biological.Macromolecule. 7-2. (1985)

    • Related Report
      1985 Annual Research Report
  • [Publications] Carrbohydrate Research. 146-1. (1986)

    • Related Report
      1985 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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