Expression of pancreatic secretory trypsin inhibitor: After surgical stress and its' significance.
| Project/Area Number |
60480303
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | OSAKA UNIVERSITY |
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Principal Investigator |
OGAWA Michio The 2nd Dept. of Surgery Osaka Univ. Medical School, Instractor, 医学部, 講師 (30028691)
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| Co-Investigator(Kenkyū-buntansha) |
中口 和則 大阪大学, 医学部・第2外科, 医員
SHIBATA Takashi The 2nd Dept. of Surgery Osaka Univ. Medical School, Surgical Staff, 医学部・第2外科, 医員
MIYAUCHI Keisuke The 2nd Dept. of Surgery Osaka Univ. Medical School, Surgical Staff, 医学部・第2外科, 医員 (70209857)
NAKAGUCHI Kazunori The 2nd Dept. of Surgery Osaka Univ. Medical School, Surgical Staff
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1986: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1985: ¥5,800,000 (Direct Cost: ¥5,800,000)
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| Keywords | pancreatic secretory trypsin inhibitor (PSTI) / インヒビター / 侵襲 / 悪性腫瘍 / 急性相蛋白 / 成長因子 |
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| Research Abstract |
Pancreatic secretory trypsin inhibitor (PSTI) was first isolated by Kazal et al. in 1948. Since then PSTI has been thought to exist only in the pancreas and to be a specific trypsin inhibitor that prevents autoactivation of trypsinogen in the pancreas and pancreatic juice. In the present project, we demonstrated that PSTI in serum is an acute phase reactant and that it increases remarkably in response to surgical stress or invasion. The magnitude of the elevation is far greater than those of acute phase reactants previously known. Serum PSTI was also elevated in patients with various malignancies. The incidence and the magnitude of the elevation of serum PSTI were far greater in patients with advanced or metastasized malignancies. By immunoperoxidase staining, we found that more than one-third of various cancer tissues contained PSTI-immunoreactive cells.
The nucleotide sequences of human PSTI mRNA and mouse epidermal growth factor (EGF) mRNA were highly homologous. Human PSTI stimulated DNA synthesis in human fibroblasts. Moreover, we revealed that PSTI bound specifically to various cultured cells, and that the binding site for PSTI was distinct from the receptor for EGF. these results suggested that the physiological role of increased PSTI in serum is related to growth stimulation for host defence against tissue destruction.
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Report
(1 results)
Research Products
(14 results)
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[Publications] 神前五郎,小川道雄(編集);小川道雄,神前五郎,北原健志,村田厚夫,森武貞,松田和彦,柴田高,松浦成昭,山本龍夫,他著: "膵分泌性トリプシン・インヒビター -基礎と臨床-" 医学図書出版(東京), 310 (1985)
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[Publications] Ogawa, M., Matsuda, K., Shibata, T., Matsuda, Y., Ukai, T., Ohta, M., Mori, T.: "Elevation of serum pancreatic secretory trypsin inhibitor (PSTI) in patients with serious injury." Res. Commun. Chem. Pathol. Pharmacol.50. 259-266 (1985)
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[Publications] Ogawa, M., Tsushima, T., Ohba, Y., Ogawa, N., Tanaka, S., Ishida, M., Mori, T.: "Stimulation of DNA synthesis in human fibroblasts by human pancreatic secretory trypsin inhibitor." Res. Commun. Chem. Pathol. Pharmacol.50. 155-158 (1985)
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[Publications] Yamamoto, T., Nakamura, Y., Nishide, T., Emi, M., Ogawa, M., Mori, T., Matsubara, K.: "Molecular cloning and nucleotide sequence of human pancreatic secretory trypsin inhibitor (PSTI) cDNA." Biochem. Biophys. Res.Commun.132. 605-612 (1985)
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[Publications] Niinobu, T., Ogawa, M., Shibata, T., Murata, A., Nishibe, S., Mori, T., Ogata, N.: "Specific binding of human pancreatic secretory trypsin inhibitor to various cultured cells." Res. Commun. Chem. Pathol. Pharmacol.53. 245-248 (1986)
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[Publications] Shibata, T., Ogawa, M., Matsuda, K., Miyauchi, K., Yamamoto, T., Mori, T.: "Purification and characterization of pancreatic secretory trypsin inhibitor in human gastric mucosa." Clinica Chimica Acta. 159. 17-36 (1986)
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[Publications] Ogawa, M., Matsuda, K., Matsuda, Y., Miyauchi, K., Nishijima, J., Horikawa, Y., Kurihara, M., Mori, T.: University of Tokyo Press. Evaluation of immunological assays of serum pancreatic enzymes and pancreatic secretory trypsin inhibitor for diagnosis and management of acute pancreatitis., 107-115 (1985)
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