The Study on the new-gingival attachment following periodontal surgery
| Project/Area Number |
60480417
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | Osaka Dental University |
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Principal Investigator |
YAMAOKA Akira Osaka Dental University, Professor, 歯学部・歯周病学講座, 教授 (30066972)
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| Co-Investigator(Kenkyū-buntansha) |
NOGUCHI Yoshihiro Osaka Dental University, Tutor, 歯学部・歯周病学講座, 助手 (80172782)
TAKADA Kohei Osaka Dental University, Tutor, 歯学部・歯周病学講座, 助手 (20103103)
NISHIMURA Kazuaki Osaka Dental University, Lecture, 歯学部・歯周病学講座, 講師 (40098017)
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| Project Period (FY) |
1985 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1986: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1985: ¥5,400,000 (Direct Cost: ¥5,400,000)
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| Keywords | Periodontal surgery / cell proliferation / 細胞増殖能 / 細胞一根面付着様式 |
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| Research Abstract |
It is suggested that the type of cells responsible for the initial repopulation to the root surface determines the results of the repair process following flap surgery. Recent studies have indicated that cells derived from the periodontal ligament, in contrast to those from the gingival connective tissue and alveolar bone, have the potential to restore periodontal architecture with new attachment. However, several studies suggested that osteogenesis enhanced the new attachment. The purpose of the present study is to determine the phenotype of cells which leads to the periodontal regeneration with new attachment.
the phenotype of cells which leads to the periodontal regeneration with new attachment.
Culture cells were isolated from the gingival connective tissue, periodontal ligament and alveolar bone by using explant method. After the three types of cells were compared in tegard to morphology(light and transmission electron microscopy)and function(doubling time, saturation density, life
… More
span)in vitro, they were each co-cultured with dentin slices in MEN+10%FBS to study on their attachment phase. Dentin slices were picked up after 1,3 and 7days of incubation.
The phase-contrast microscopic findings showed that the emigrating culture cells from gingiva and periodontal ligament appeared to be fibroblastic, while culture cells from alveolar bone had mosaic appearance. Little difference in ultrastructural features was found among the three types of cells, but cell junction and extracellular microfibrils were observed in cells from alveolar bone only. The log of growth curve showed that cells from periodontal ligament were inferior to those from gingiva and alveolar bone in the proliferation and migration potency. Concerning the attachment-activity to the root surface, cells from alveolar bone were superior to those from gingival and periodontal ligament.
These findings suggest that the osteoblast-like cells which appeared to the coronal root surface could,at least in part, be concerned in the new cementum formation in a limited portion of supercrestal area. Less
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Report
(2 results)
Research Products
(6 results)
