Artifical crossing of pufferfishes to examination the origin of natural hybrids and to find crosses suitable for aquaculture purpose

Research Project

Project/Area Number	60560202
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	General fisheries
Research Institution	Nagasaki University
Principal Investigator	DOTSU Yoshie Faculty of Fisheries, Nagasaki University; Professor, 水産学部, 教授 (50039715)
Project Period (FY)	1985 – 1986
Project Status	Completed (Fiscal Year 1986)
Budget Amount *help	¥1,600,000 (Direct Cost: ¥1,600,000) Fiscal Year 1986: ¥500,000 (Direct Cost: ¥500,000) Fiscal Year 1985: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywords	Pufferfishes / Wild hybrids / Crosses by cryopreservative spermatozoon / Culture of artifical crosses / Characters of artificial crosses / 交雑フグの養殖適種
Research Abstract	Five types of pufferfish, supposed to be hybrid, had been caught from the Japanese seas. Possible parents of each hybrid were speculated through examination of the fishes on the internal and external characters including electrophoretic characters. To verify the speculation 20 combinations of interspecific cross experiments carried out among following 8 species of pufferfishes; Takifugu rubripes, T. chinensis, T. porohyreus, T. wanthopterus, T. niphobles, T. radiatus, T. pardalis and T. poecilonotus by natural and cryopreservative spermatozoa. Larvae of 7 crosses were cultured to young or adult stages. The crosses of rubripes <+!゜> X chinensis <^!゜> , rubripes <+!゜> X xanthopterus <^!゜> niphobles <+!゜> X rubripes <^!゜> and pardalis <+!゜> X rubripes <^!゜> looked their mothers in color and pattern of body; pardalis <+!゜> X rublipes <^!゜> and niphobles <+!゜> X rubripes <^!゜> like the fathers; and some of rubripes <+!゜> X poecilonotus <^!゜> like the mother and others like the father. The characters of the artifical hybrids did not give the clue of verify parents of the natural hybrids without doubt. None of the artifical crosses tested was found to be better for aquaculture than the tiger puffer Takifugu rubripes which is cultured commercially.