| Project/Area Number |
60560203
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General fisheries
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| Research Institution | Faculty of Agriculture, Miyazaki University |
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Principal Investigator |
AOKI Takashi Faculty of Agriculture, Miyazaki University, 農学部, 助教授 (00051805)
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| Co-Investigator(Kenkyū-buntansha) |
KITAO Tadatoshi Faculty of Agriculture, Miyazaki University, 農学部, 教授 (60094075)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1986: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1985: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | fish / carp / yellowtail / globin gene / nucleotide sequence of <alpha> -globin / restriction map |
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| Research Abstract |
Genomic clones that contain the <alpha> -globin of the marine fish yellowtail (seriola quinqueradiata) were isolated by cross-hybridization to <alpha> -globin cDNA of carp from the yellowtail genomic library. The <alpha> -globin gene of yellowtail with the length of 1.6 K bases was cloned. The endonuclease restriction map of <alpha> -globin gene of yellowtail was almost similar to <alpha> -globin gene of carp. we determined the complete nucleotide sequences of the <alpha> -globin gene of carp containing 2 introns and 3 exons. The length of the <alpha> -globin gene from the codon initiating translation(ATG) to the terminal codon(TAA) was 714 base pairs(bp). From the 5' leader sequences to the 3' untranslated regions, the first 96 bp nucleotide of the <alpha> -globin gene are encoded in a single exon, followed by intron 1, a second exon domain extending to codon 264, a second intron, and a third exon domain(134 pb) comprising the rest of the transcribed DNA. The first intron(176 bp) of <alpha> -globin is slightly larger than the second intron(106 bp). Similar sequences are found in the 3' untranslated region and the introns in correponding locations in most mammalian <alpha> -globin genes.
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