Production of Interspecific Chimeric Blastocysts.
Project/Area Number |
60560271
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Research Category |
Grant-in-Aid for General Scientific Research (C)
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Allocation Type | Single-year Grants |
Research Field |
畜産学(含草地学)
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Research Institution | Iwate University |
Principal Investigator |
TAKAHASHI Jutaro Derartment of Agriculture, Iwate University, Associate Professor, 農学部, 助教授 (00004929)
|
Project Period (FY) |
1985 – 1986
|
Project Status |
Completed (Fiscal Year 1986)
|
Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1986: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1985: ¥1,400,000 (Direct Cost: ¥1,400,000)
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Keywords | Interspecific chimeras / Development of embryos / Culture of embryo in vitro / キメラ胚盤胞 |
Research Abstract |
The production of interspecific chimeric embryos may be available for analysis of the " capacity " of embryos in early developmental stages. As a culture medium, Dulbecco's modified eagle medium with 0.3 % bovine serum albumin ( pH 7.4 ) was used for the production of xenogeneic chimeric embryos ( Mouse:Rat, Mouse:Hamster, Rat:Hamster ). From a total of 53 aggregates of a mouse embryo with a rat embryo, 43 ( 81.1% ) developed into chimeric blastocysts, and this rate was similar to those of the Mouse:Mouse and the Rat--Rat systems. In the Hamster:Hamster and the Rat:Hamster systems, no chimeric blastocyst was obtained. However, in combination of mouse and hamster embryos, only 3 of 33 aggregates developed into chimeric blastocysts. These results may indicate that there is a great discrepancy between families in developmental biology.
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Report
(1 results)
Research Products
(5 results)