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BASIC RESEARCH FOR THE FUSION AND DESTRUCTION OF LIPOSOMES BY PHOTOACTIVATION

Research Project

Project/Area Number 60870089
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field 医学一般
Research InstitutionKyoto University

Principal Investigator

ORII YUTAKA  FACULTY OF MEDICINE, KYOTO UNIVERSITY, 医学部, 助教授 (60028149)

Co-Investigator(Kenkyū-buntansha) KAWANISHI SHOSUKE  FACULTY OF MEDICINE, KYOTO UNIVERSITY, 医学部, 講師 (10025637)
SANO SEIYO  FACULTY OF MEDICINE, KYOTO UNIVERSITY, 医学部, 教授 (60025533)
Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥9,900,000 (Direct Cost: ¥9,900,000)
Fiscal Year 1986: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1985: ¥8,800,000 (Direct Cost: ¥8,800,000)
KeywordsFUSION OF LIPOSOMES / DESTRUCTION OF LIPOSOMES / PORPHYRIN LIPOSOMES / DYE LASER / リポソーム破壊 / 光励起 / チトクロム酸化酵素リポソーム / 光散乱強度 / 電導度
Research Abstract

BASIC EXPERIMENTS FOR THE FUSION AND DESTRUCTION OF LIPOSOMES WERE CARRIED OUT AS FOLLOWS.
1. A LARGE SCALE PREPARATION METHOD OF PROTEOLIPOSOMES WAS DEVELOPED BY INTRODUCING THE DIALYSIS PROCEDURE.
2. A DYE LASER FLASH PHOTOLYSIS APPARATUS WAS CONSTRUCTEDINCORPORATING INTERFACES FOR A PERSONAL COMPUTOR. A DEVICE CONTROL AND DATA ANALYSES COMPUTOR PROGRAMS WERE PREPARED BY USING C LANGUAGE.
3. SPECTROPHOTOMETRIC AND ELECTROCHEMICAL PROCEDURES WERE DEVISED TO MONITOR THE DESTRUCTION OF LIPOSOMES.
4. WHEN BOTH BACTERIORHODOPSIN AND CYTOCHROME OXIDASE LIPOSOMES WERE FUSED WITH CALCIUM ION, A RELEASE OF RESPIRATION WAS OBSERVED WHEN IRRADIATED. THIS EXTENT WAS UTILIZED TO MONITOR THE FUSION OF LIPOSOMES.
5. LIPOSOMES RECONSTITUTED WITH PROTOPORPHYRIN WAS DESCTRUCTED RAPIDLY WHEN IRRADIATED, ITS EXTENT BEING DEPENDENT ON THE CONCENTRATION OF OXYGEN IN THE MEDIUM. UNDER NITROGEN THE DESTRUCTION WAS INHIBITED TO A LARGE EXTENT.
6. THE ENZYMIC ACTIVITY OF LIPOSOMES RECONSTITUTED WITH CYTOCHROME OXIDASE AND PROTOPORPHYRIN WAS INHIBITED ALMOST COMPLETELY BY IRRADIATION.
7. PHOTOEXCITATION OF PORPHYRINLIPOSOMES AND ITS DECAY WERE ANALYZED BY THE FLASH-PHOTOLYSIS METHOD REVEALING THE BIPHASIC DECAY OF THE EXCITED STATE. THE DECAY WAS ALSO DEPENDENT ON THE OXYGEN CONCENTRATION.

Report

(2 results)
  • 1986 Final Research Report Summary
  • 1985 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] 三木俊明: J.Biol.Chem.261. 3915-3918 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 三木俊明: J.Biochem.100. 735-745 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 折井豊: J.Biol.Chem.261. 3544-3547 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 三木俊明: J.Biochem.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 折井豊: 生物物理. 26. S283 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 折井豊: 準備中.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 折井豊: "チトクロム酸化酵素リポソームのチトクロムCペルオキシダーゼ活性とプロトンポンプ in "Membrane Biochemistry and Bioenergetics"" Plenum Press,New York, (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 佐野晴洋: "ポルフィリン症(蛋白質核酸・酵素別冊「血色素の分子生理と分子病理」)" 共立出版, (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] TOSHIAKI MIKI: "PROTON TRANSLOCATION BY CYTOCHROME OXIDASE VESICLES CATALYZING THE PEROXIDATIC OXIDATION OF FERROCYTOCHROME C." J. BIOL. CHEM.261. 3915-3918 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] TOSHIAKI MIKI: "CYTOCHROME C PEROXIDASE ACTIVITY OF BOVINE HEART CYTOCHROME OXIDASE INCORPORATED IN LIPOSOMES AND GENERATION OF MEMBRANE POTENTIAL." J. BIOCHEM.100. 735-745 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] YUTAKA ORII: "PHOTODISSOCIATION OF OXYGENATED CYTOCHROME O(S) (VITREOSCILLA) AND KINETIC STUDIES OF REASSOCIATION." J. BIOL. CHEM.261. 3544-3547 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] TOSHIAKI MIKI: "A MECHANISM OF RESPIRATORY CONTROL: STUDIES WITH PROTEOLIPOSOMES CONTAINING CYTOCHROME OXIDASE AND BACTERIORHODOPSIN" J. BIOCHEM.(1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] YUTAKA ORII: "MEASUREMENTS OF THE TRANSITIONAL STAGE OF PROTON TRANSLOCATION BY FUNCTIONAL LIPOSOMES." BIOPHYSICS. 26. S283 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] YUTAKA ORII: PLENUM PRESS, NEW YORK. CYTOCHROME C PEROXIDASE ACTIVITY OF LIPOSOMAL CYTOCHROME OXIDASE AND ITS COUPLING TO PROTON PUMPING. IN "MEMBRANE BIOCHEMISTRY AND BIOENERGETICS", (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] ANALYTICAL BIOCHEM.146. (1985)

    • Related Report
      1985 Annual Research Report
  • [Publications] 生化学. 57-8. (1985)

    • Related Report
      1985 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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