| Project/Area Number |
61480160
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Immunology
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| Research Institution | Juntendo University |
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Principal Investigator |
OKUMURA Ko Professor, Juntendo Univ., Sch. of Medicine, 医学部, 教授 (50009700)
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| Co-Investigator(Kenkyū-buntansha) |
YAGITA Hideo Associate Professor, Juntendo Univ., Sch. of Medicine, 医学部, 助教授 (30182306)
NAKAUCHI Hiromitsu Associate Professor, Juntendo Univ., Sch. of Medicine, 医学部, 非常勤講師 (40175485)
KUMAGAI Yoshihiro Associate Professor, Juntendo Univ., Sch. of Medicine, 医学部, 非常勤講師 (80175314)
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| Project Period (FY) |
1986 – 1988
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| Project Status |
Completed (Fiscal Year 1988)
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| Budget Amount *help |
¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 1988: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1987: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1986: ¥3,100,000 (Direct Cost: ¥3,100,000)
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| Keywords | LFA / Ts / Monoclonal Antibody / CD8 / CD2 / パーフォリン / トランスフェクション / CD8遺伝子 / CD2遺伝子 / リンパ球機能分子 / トランスフェクタント / FACS / DNAクローニング / サプレッサーT細胞 / T細胞レセプター / T細胞機能分子 |
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| Research Abstract |
Including suppressor, helper and killer T cell, many kinds of lymphocyte function associated antigen(LFA) has been know to play important role for their differentiation and activation. In this project, experiments were performed to characterize the function of LFAs by using cDNA coding LFAs and monoclonal antibodies reactive with LFAs. Addition to CD8, murine CD2 cDNA was cloned and the monoclonal antibody was made. In the well characterized mouse experimental system, the role of CD2 was examined in comparison with the notion obtained from human T cells experiments. Several important differences were observed in terms of their distribuation and functions comparing to human systems. These finding were thought to be extremely useful to understand the role of LFAs in immune regulation. Beside LFAs study, the final effector molelcules of killer lymphocytes has also been studied from molecular level. cDNA cording perforin was cloned and the role of perforin was extensively studies by using cloned cDNA and various new findingds were abserved in mouse, rat and human killer systems.
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