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The role of Japositive denditc cells in organ graht rejection

Research Project

Project/Area Number 61480227
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Dermatology
Research InstitutionYamanashi Medical College (1988)
The University of Tokyo (1986-1987)

Principal Investigator

TAMAKI Kunihiko  Yamanashi Medical College Professor, 教授 (30010432)

Co-Investigator(Kenkyū-buntansha) IIJIMA Masafumi  Showa University, School of Medicine, 医学部, 助教授 (20010449)
NAKAMURA Koichiro  Branch Hospital, Faculty at Mcdicine, University of Tokyo, 医学部, 医員 (60175502)
石井 晶子  東京大学, 医学部, 医員
SEKI Yoshihito  Branch Hospital, Faculty of Medicine, University of Tokyo, 医学部, 講師 (50114525)
ISHII Akiko  Branch Hospital, Faculty at Mcdicine, University of Tokyo
Project Period (FY) 1986 – 1988
Project Status Completed (Fiscal Year 1988)
Budget Amount *help
¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 1988: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1987: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1986: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsSkin graft rejection / Delayet-type hyperscnsitirty / Cytofoxic T lrynphoeyte / 表皮細胞 / ランゲルハンス細胞 / UVB / 臓器移植 / Ia抗原陽性樹枝状細胞 / アロ抗原 / 遅延型過敏症
Research Abstract

Althcugh in vitro role of epiderwal Langeilans cells (LC) has been studied extensitiely in vivo rile of LC is still a matter of controversy. In a preions paper, we have estellishied a precise condition to induce delayed-type hypersenoiticrty (DTH) reaction to alloantigens using epidermal cells (EC). In order to clauty the role of LC in vivo, we first used UVB inadiation for the reduction of this DTH reaction. The UVB effect on the induction of cytotoxic T lymphocyte activity (CTL) in vivo and on shingraft nrejection was also mivestigated. Aterthe shin was iisradiated with UVB in vitro, EC were obtainecl. These EC were iinjected via subcutanevus (DC) route and DTH reaction was compared to that mentreated EC. Reduction of DTH seaction who obsewed (fron 62 to 99.1%). CTL activity in these mice was assessed aften in vitrs stimulation. CTL actiorty in miee sensitized with UVB inadiated EC was apprecially seduced. Furtheimore mice scmityod with upb irsadiated EC did not reject the followingon-uvb-inadiated ec rejected auhsegnent shin allograftin an accelewthe feshion
Next we used balb/c shin transpleuted onto (cbhxbacr/c)fimice, these jrefted plrin was taken 150 days aftir trimsplantation and ec were made, these ec were injected sc into mormil balb/c mice. The role of lc was studied by depleting lc with anti ta^k serum and c rnplement treatinent. For dth reaction significent reduction was notcd in anti ta^k serum + conplement treated mice. for ctl activity, appaeciable reduction who noted in anti-ta^k + conplement treated group. when uvb wao used reduced tdh reuction and morwel ctl activity was noticed. thus althcugh lc was necessauy for both dth and ctl induction, other mechauism wuned be operatig when uvbv irrediation was used.

Report

(4 results)
  • 1988 Annual Research Report   Final Research Report Summary
  • 1987 Annual Research Report
  • 1986 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] 玉置邦彦: 日本皮膚科学会雑誌. 97. 13-46 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 玉置邦彦: Jpn J Clin Jmnurn. 11. 446 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kunihiko Tamaki,;Masafumi Iijima: Trensplantation.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kunihiko Tamaki,;Masafumi Iijima: J Invest Dermatol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] 玉置邦彦: "ランゲルハンス細胞 in Immunchematology" 近代出版,

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kunihiko, Tamki: "T cell mediated immunity in shin graft reyeation" The Japanese Journal of Deumahlogy. 97. 1346 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kunihiko, Tamki: "Jangerhaus cells and delayed-type hypersenitirty" Japanese Journal of Clinical Immunology. 11. 446 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kunihiko Tamaki; Masafumi Iijima: "The effect of ultrauolet B iradiation on delayed-type hynersenitioity, cytotoxic T lymphocyte actirty and shin graft rejection" Transplantation.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kunihiko Tamaki; Masafumi Iijima: "Langeihaus cells are reguired for bolb induction of delayed-type hypersensiticrty and for cytotoxic T lymphocyto induction to alloantigens by epidermal cells" Journal for Inicstigatice Dermatology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Koichiro Nakamura; Akiko Ishii; Kunihiko Tamaki: "Sulpopulation and their migration prifles of murise epiderwel Langerhons cells identified by lectin binding sites"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kunihiko Tamaki: Langerhons cell. Kindai Syuppan, (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1988 Final Research Report Summary
  • [Publications] Kunihiko Tamaki;Masafumi Iijima: J Invest Dermatol.

    • Related Report
      1988 Annual Research Report
  • [Publications] 玉置邦彦: "Imnumhematology" 近代出版,

    • Related Report
      1988 Annual Research Report
  • [Publications] Kunihiko Tamaki;etal: clirncal Research. (1988)

    • Related Report
      1987 Annual Research Report
  • [Publications] Kunihiko Tamaki;Masutaha Fune: J Invest Deunatol.85. 20-24 (1985)

    • Related Report
      1986 Annual Research Report
  • [Publications] 玉置邦彦: 日皮会誌. 97. (1987)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2017-04-05  

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